| 产品编号 | bs-0394R |
| 英文名称 | HOXc8 |
| 中文名称 | HOXc8抗体 |
| 别 名 | Hox-3.1; Homeo box 3A; Homeo box 8; Homeobox 3A; Homeobox C8; Homeobox protein Hox C8; Homeobox protein Hox-C8; Homeobox protein HoxC8; Homeobox protein R4; Homolog of mouse Hox 3.1; HOX 3; Hox 3.1 mouse homolog of; HOX 3A; HOX C8; Hox-3.1, MOUSE, HOMOLOG OF; Hox-3A; HOX3; HOX3A; HOXC 8; Hoxc-8; M31; HXC8_HUMAN. |
| 研究领域 | 免疫学 神经生物学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HOXc8: 51-130/242 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene may play a role in the regulation of cartilage differentiation. It could also be involved in chondrodysplasias or other cartilage disorders. Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Subunit: Interacts with SMAD1 and HOMEZ. Subcellular Location: Nucleus. Similarity: Belongs to the Antp homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P31273 Gene ID: 3224 Database links: Entrez Gene: 3224 Human Entrez Gene: 15426 Mouse Omim: 142970 Human SwissProt: P31273 Human SwissProt: P09025 Mouse Unigene: 664500 Human Unigene: 6167 Mouse HOXc8基因是HOX基因家族中的一员，研究小鼠中HOXc8蛋白的位置、结构以及HOXc8蛋白在小鼠胚胎发育中的表达与调控、小鼠神经系统的发育均已被广泛重视. HOXc8蛋白的表达对APP基因的表达有抑制作用,有学者认为HOXc8基因可能与Alzheimer's病的发病有关. 目前对哺乳动物同源盒基因在中枢神经系统发育中的广泛表达以及在胚胎发育中的区域特异性表达方式被认为是非常重要的，已引起科学家们的广泛重视。 |
| 产品图片 |
Sample:
Brain(Rat) lysate at 30ug; Liver(Rat) lysate at 30ug; Primary: Anti-HOXc8 (bs-0394R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; Predicted band size : 27kD Observed band size : 27kD, 43kD 
Sample:
Liver(Rat) Lysate at 40 ug Primary: Anti-HOXc8 (bs-0394R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD 
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3) Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with (Hox-3.1) Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
