| 产品编号 | bs-1108R |
| 英文名称 | IGFBP2 |
| 中文名称 | 胰岛素样生长因子结合蛋白2抗体 |
| 别 名 | BP 2; BP2; IBP 2; IBP-2; IBP2; IBP2_HUMAN; IGF binding protein 2; IGF BP53; IGF-binding protein 2; IGFBP 2; IGFBP-2; IGFBP2; IGFBP53; Insulin like growth factor binding protein 2 36kDa; Insulin like growth factor binding protein 2; Insulin like growth factor-binding protein 2 precursor; Insulin-like growth factor-binding protein 2. |
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Specific References (3) | bs-1108R has been referenced in 3 publications.
[IF=6.792] Zhao D et al. PCB52 exposure alters the neurotransmission ligand-receptors in male offspring and contributes to sex-specific neurodevelopmental toxicity. Environ Pollut.2020 Sep;264:114715. WB ; Rat.
[IF=3.464] Xiubing Chen. et al. Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma. DIS MARKERS. 2023 Jan 20;2023:5145152 IHC ; Human.
[IF=2.776] Liu Y et al. IGFBP2 promotes immunosuppression associated with its mesenchymal induction and FcγRIIB phosphorylation in glioblastoma. PLoS One. 2019 Sep 27;14(9):e0222999. IHC-P&WB&FCM ; Mouse.
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| 研究领域 | 肿瘤 免疫学 生长因子和激素 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IGFBP2: 101-200/305 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
IGFBP2 (insulin-like growth factor binding protein 2) is a member of the ISGBP family which bind various IGFs. IGFBP2 is overexpressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The overexpression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness Function: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Subcellular Location: Secreted. Post-translational modifications: O-glycosylated. Similarity: Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain. SWISS: P47877 Gene ID: 3485 Database links: Entrez Gene: 3485 Human Entrez Gene: 16008 Mouse Omim: 146731 Human SwissProt: P18065 Human SwissProt: P47877 Mouse SwissProt: Q91VK7 Mouse Unigene: 438102 Human Unigene: 141936 Mouse Unigene: 6813 Rat 生长因子和激素（ Growth Factor and Hormones） 胰岛素样生长因子结合蛋白-2(IGFBP-2)是IGF/IGFBP家族中的一个重要成员,在多种疾病尤其是肿瘤中其表达明显增高,可能通过调节胰岛素样生长因子(insulin-like growth factor,IGF)发挥作用，促进肿瘤的发生发展。 |
| 产品图片 |
Sample:
Lane 1: Human MOLT4 cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-IGFBP2 (bs-1108R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa 
Sample:
Liver (Mouse) Lysate at 40 ug Primary: Anti-IGFBP2 (bs-1108R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD 
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP2 Polyclonal Antibody, Unconjugated(bs-1108R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP2 Polyclonal Antibody, Unconjugated(bs-1108R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
