产品中心-北京博奥森生物技术有限公司

产品编号	bs-0172R
英文名称	IRS1
中文名称	胰岛素受体底物-1抗体
别名	IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;
	Specific References (16) \| bs-0172R has been referenced in 16 publications. [IF=7.129] Yanwen Hou. et al. Prenatal PM2.5 exposure contributes to neuronal tau lesion in male offspring mice through mitochondrial dysfunction-mediated insulin resistance. ECOTOX ENVIRON SAFE. 2022 Nov;246:114151 WB ; Mouse. PubMed:36228359 [IF=7.097] Yilan Shen et al. VEGF-B antibody and interleukin-22 fusion protein ameliorates diabetic nephropathy through inhibiting lipid accumulation and inflammatory responses. IHC ; mouse. PubMed:10.1016/j.apsb.2020.07.002 [IF=7.097] Yilan Shen et al. VEGF-B antibody and interleukin-22 fusion protein ameliorates diabetic nephropathy through inhibiting lipid accumulation and inflammatory responses. PubMed:10.1016/j.apsb.2020.07.002 [IF=6.796] Tingting Ku. et al. Cardiac energy metabolism disorder mediated by energy substrate imbalance and mitochondrial damage upon tebuconazole exposure. J ENVIRON SCI-CHINA. 2022 Oct;: WB ; Mouse. PubMed:10.1016/j.jes.2022.10.012 [IF=6.691] Wang, Hongyan. et al. Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways. 2021 Aug 28 WB ; Rat. PubMed:34455488 [IF=6.304] Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14 WB ; Bovine. PubMed:33542215 [IF=6.208] Xuan Hu. et al. Effects of Chicken Serum Metabolite Treatment on the Blood Glucose Control and Inflammatory Response in Streptozotocin-Induced Type 2 Diabetes Mellitus Rats. INT J MOL SCI. 2023 Jan;24(1):523 IHC ; Chicken. PubMed:36613966 [IF=4.225] Chen Xuan. et al. Yiqi Jiedu Huayu Decoction Alleviates Renal Injury in Rats With Diabetic Nephropathy by Promoting Autophagy. Front Pharmacol. 2021; 12: 624404 WB ; Rat. PubMed:33912044 [IF=4.221] Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192 WB ; Mouse. PubMed:35489422 [IF=3.69] Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947 WB ; Rat. PubMed:33617969 [IF=3.098] Zhang et al. Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. (2018) Molecules. 23 WB ; Mouse. PubMed:30347867 [IF=2.723] Cao, Hui. et al. 14-3-3β is essential for milk composition stimulated by Leu/IGF-1 via IGF1R signaling pathway in BMECs. IN VITRO CELL DEV-AN. 2022 Jun;:1-12 WB ; Bovine. PubMed:35648337 [IF=2.018] Yan X et al. Antidiabetic potential of green seaweed Enteromorpha prolifera flavonoids regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice. (2018) J Food Sci. WB ; Mouse. PubMed:30569533 [IF=1.832] Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193. WB ; ram lambs. PubMed:31022943 [IF=1.81] Dong, Peiyue, et al. "MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1." Acta Biochimica et Biophysica Sinica (2014): gmu043. WB ; Pig. PubMed:24862853 [IF=1.513] Ting Zhang. et al. Sea buckthorn ( Hippophae rhamnoides L.) oil enhances proliferation, adipocytes differentiation and insulin sensitivity in 3T3-L1 cells. Food Sci Biotechnol. 2020 Nov;29(11):1511-1518 WB ; Mouse. PubMed:33088600
研究领域	细胞生物神经生物学信号转导细胞凋亡糖尿病内分泌病
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat,Pig (predicted: Chicken)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	137kDa
细胞定位	细胞核细胞浆细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from rat IRS-1: 1101-1200/1242
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed. Function: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Subunit: Interacts with UBTF and PIK3CA. Interacts (via phosphorylated YXXM motifs) with PIK3R1. Interacts with ROCK1 and FER. Interacts (via PH domain) with PHIP. Interacts with GRB2. Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK. Subcellular Location: IRS1 is predominantly found in the cytoplasm. Nuclear localization may occur in some cell types and under specific stimuli. Post-translational modifications: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding. DISEASE: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) Similarity: Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: P35568 Gene ID: 3667 Database links: Entrez Gene: 3667 Human Entrez Gene: 16367 Mouse Entrez Gene: 25467 Rat SwissProt: P35568 Human SwissProt: P35569 Mouse SwissProt: P35570 Rat 信号传导（Signaling Intermediates）胰岛素受体底物-1是细胞中分子量为165-185kDa的磷蛋白，胰岛素受体的内源性底物。经胰岛素刺激后其酪氨酸残基被磷酸化。
产品图片	Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRS1) Polyclonal Antibody, Unconjugated (bs-0172R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRS1) Polyclonal Antibody, Unconjugated (bs-0172R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记