产品中心-北京博奥森生物技术有限公司

产品编号	bs-0821R
英文名称	Laminin subunit beta-1
中文名称	层粘连蛋白β1抗体
别名	LAMB1; Laminin B1 chain; Laminin subunit beta 1; Laminin-1 subunit beta; Laminin-10 subunit beta; Laminin-12 subunit beta; Laminin-2 subunit beta; Laminin-6 subunit beta; Laminin-8 subunit beta;LAMB1_HUMAN.
	Specific References (11) \| bs-0821R has been referenced in 11 publications. [IF=11.092] Guobao Chen. et al. In Situ Regulation and Mechanisms of 3D Matrix Stiffness on the Activation and Reversion of Hepatic Stellate Cells. ADV HEALTHC MATER. 2022 Dec;:2202560 IF ; Rat. PubMed:36519640 [IF=8.32] Gong, Tao, et al. "A Dynamically Tunable, Bioinspired Micropatterned Surface Regulates Vascular Endothelial and Smooth Muscle Cells Growth at Vascularization." Small (2016). IHC-P ; Rabbit. PubMed:27595865 [IF=5.959] Wang Y et al. SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway. Cell Death Dis. 2019 Nov 7;10(11):852. IHF ; Mouse. PubMed:31699966 [IF=5.116] Siwen Zhang. et al. Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Stem Cell Res Ther. 2021 Dec;12(1):1-16 IF,IHC ; Rat. PubMed:33712079 [IF=4.831] Zhang Q et al. CTRP13 attenuates the expression of LN and CAV-1 Induced by high glucose via CaMKKβ/AMPK pathway in rLSECs. Aging (Albany NY). 2020 Jun 17;12(12):11485-11499. WB ; Rat. PubMed:32554851 [IF=4.2] Miyauchi, Yuya, et al. "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." Scientific Reports 7 (2017). IHC-P ; Rat. PubMed:28851916 [IF=4.19] Su, Chuanyang, et al. "Tetrachlorobenzoquinone activates Nrf2 signaling by Keap1 cross-linking and ubiquitin translocation, but not Keap1-Cullin3 complex dissociation." Chemical Research in Toxicology (2015). WB ; Human. PubMed:25742418 [IF=4.146] Jian Kang. et al. Ginsenoside Rg1 Mitigates Porcine Intestinal Tight Junction Disruptions Induced by LPS through the p38 MAPK/NLRP3 Inflammasome Pathway. TOXICS. 2022 Jun;10(6):285 WB ; Mouse,Pig. PubMed:10.3390/toxics10060285 [IF=3.701] S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062. IHC-P ; Rat. PubMed:doi:10.1016/j.jff.2020.104062 [IF=1.81] Wang Hua. et al. Distribution of extracellular matrix related proteins in normal and cryptorchid ziwuling black goat testes. ANIM REPROD. 2022 Jun;19: IHC ; Goat. PubMed:35712443 [IF=0] Ijima H et al. Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver. Gels, 2018 4(2), 39. IHF ; Pig. PubMed:10.3390/gels4020039
研究领域	肿瘤细胞生物免疫学神经生物学信号转导细胞凋亡
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Rat,Mouse,Human (predicted: Cow)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	198kDa
细胞定位	细胞膜细胞外基质分泌型蛋白
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Laminin subunit beta-1: 901-1000/1786
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq, Aug 2011] Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface. Subcellular Location: Secreted > extracellular space > extracellular matrix > basement membrane. Tissue Specificity: Broadly expressed in: skin, heart, lung, and the reproductive tracts. SWISS: P07942 Gene ID: 3912 Database links: Entrez Gene: 10319 Human Entrez Gene: 284217 Human Entrez Gene: 3908 Human Entrez Gene: 3912 Human Entrez Gene: 3913 Human Entrez Gene: 3915 Human Entrez Gene: 3918 Human Entrez Gene: 16772 Mouse Entrez Gene: 316758 Rat Omim: 150320 Human SwissProt: P07942 Human SwissProt: P24043 Human SwissProt: P25391 Human SwissProt: P55268 Human SwissProt: Q9Y6N6 Human SwissProt: P19137 Mouse SwissProt: Q9R0B6 Mouse Unigene: 201805 Human Unigene: 270364 Human Unigene: 302362 Mouse 层粘连蛋白主要存在于基膜(basal lamina)结构中，是基膜所特有的非胶原糖蛋白。层粘连蛋白在细胞发育过程中刺激细胞粘着、细胞运动。LN能够刺激胚胎中神经轴的生长,并促进成年动物的神经损伤后重生长和再生。如同纤粘连蛋白,细胞外的LN能够影响细胞的生长、迁移和分化。LN在原生殖细胞的迁移中起关键作用。层粘连蛋白是细胞外基底膜的主要组成部分，分布于基底膜和细胞外基质内、成纤维细胞、内皮细胞和平滑肌细胞分泌层粘连蛋白。肿瘤的浸润和转移与其遭到破坏相关。该抗体可用于标记基底膜，有助于观察肿瘤周围基底膜的分布及其改变，间接提示该肿瘤的分化程度和浸润情况。
产品图片	Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Skin (Mouse) Lysate at 40 ug Lane 3: Placenta (Mouse) Lysate at 40 ug Lane 4: U2os (Human) Cell Lysate at 30 ug Lane 5: Huvec (Human) Cell Lysate at 30 ug Lane 6: HepG2 (Human) Cell Lysate at 30 ug Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 8: A549 (Human) Cell Lysate at 30 ug Lane 9: U251 (Human) Cell Lysate at 30 ug Primary: Anti-laminin (bs-0821R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 220-240 kD Observed band size: 240 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (bs-0821R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (bs-0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (bs-0821R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Transplantation tumor of In nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-laminin Polyclonal Antibody, Unconjugated(bs-0821R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记