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Rabbit Anti-CD11b  antibody (bs-1014R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1014R
英文名称 CD11b
中文名称 整合素αM/巨噬细胞表面分子抗体
别    名 Antigen CD11b p170; CD11 antigen like family member B; CD11b antigen; CD49d; Cell surface glycoprotein MAC 1 alpha subunit; Cell Surface Glycoprotein MAC1 Alpha Subunit; Complement Component Receptor 3 Alpha; CR 3 alpha chain; CR3 Alpha Chain; CR3A; Integrin Alpha M; integrin alpha M complement component 3 receptor 3 subunit; Leukocyte Adhesion Receptor MO1; MAC 1; MAC1A; MGC117044; MO1A; Neutrophil Adherence Receptor; CD11b/CD18; F730045J24Rik; Ly-40; ITGAM; CR3A;complement receptor type 3; CR3; ITAM_HUMAN.  Integrin αM; Integrin α M.
Specific References  (41)     |     bs-1014R has been referenced in 41 publications.
[IF=41.577] Yamaguchi T et al. "Interspecies organogenesis generates autologous functional islets." Nature. 2017 Feb 9;542(7640):191-196.  IHF ;  Mouse&Rat.  
[IF=30.849] Yao Li. et al. Rapid surface display of mRNA antigens by bacteria-derived outer membrane vesicles for a personalized tumor vaccine. 2022 Mar 22  IF ;  Mouse.  
[IF=15.304] Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683  IHC, IF ;  Rat.  
[IF=12.121] Shanshan Hu. et al. A mussel-inspired film for adhesion to wet buccal tissue and efficient buccal drug delivery. Nat Commun. 2021 Mar;12(1):1-17  IHC ;  Rat.  
[IF=9.776] Ruijing Zhao. et al. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022 May;345:214  FC ;  Human.  
[IF=9.587] Li, Qiao-Yun. et al. Neuroinflammation in the anterior cingulate cortex: the potential supraspinal mechanism underlying the mirror-image pain following motor fiber injury. J NEUROINFLAMM. 2022 Dec;19(1):1-20  WB ;  Rat.  
[IF=8.821] Ning Ran. et al. MOTS‐c promotes phosphorodiamidate morpholino oligomer uptake and efficacy in dystrophic mice. Embo Mol Med. 2021 Feb;13(2):e12993  IHC ;  Mouse.  
[IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673  IHC ;  Rat.  
[IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359  IF ;  Mouse.  
[IF=6.656] Vittoria Borgonetti. et al. Zingiberene, a non-zinc-binding class I HDAC inhibitor: A novel strategy for the management of neuropathic pain. PHYTOMEDICINE. 2023 Mar;111:154670  IF ;  Mouse.  
[IF=6.63] Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17  IHC ;  Mouse.  
[IF=6.6] Prasant Kumar Jena. et al. Intestinal Microbiota Remodeling Protects Mice from Western Diet-Induced Brain Inflammation and Cognitive Decline. Cells-Basel. 2022 Jan;11(3):504  WB ;  Mouse.  
[IF=6.388] Vittoria Borgonetti. et al. Cannabidiol-rich non-psychotropic Cannabis sativa L. oils attenuate peripheral neuropathy symptoms by regulation of CB2-mediated microglial neuroinflammation. PHYTOTHER RES. 2022 Dec;:  IF ;  Mouse.  
[IF=6.304] Feng Y et al. Long noncoding RNA HOXA-AS2 functions as an oncogene by binding to EZH2 and suppressing LATS2 in acute myeloid leukemia (AML)Cell Death Dis.2020 Dec 2;11(12):1025.  IHC ;  Mouse.  
[IF=6.17] Ying Xu. et al. PINK1-mediated mitophagy protects against hepatic ischemia/reperfusion injury by restraining NLRP3 inflammasome activation. Free Radical Bio Med. 2020 Nov;160:871  IF,IHC ;  Mouse.  
[IF=5.893] Vittoria Borgonetti. et al. Combined inhibition of histone deacetylases and BET family proteins as epigenetic therapy for nerve injury-induced neuropathic pain. Pharmacol Res. 2021 Jan;:105431  IF ;  Mouse.  
[IF=5.714] Ruyi Qu. et al. Glucocorticoids improve the balance of M1/M2 macrophage polarization in experimental autoimmune uveitis through the P38MAPK-MEF2C axis. INT IMMUNOPHARMACOL. 2023 Jul;120:110392  FCM ;  Rat.  
[IF=5.712] Bronzuoli MR et al. Neuroglia in the autistic brain: evidence from a preclinical model. Mol Autism. 2018 Dec 27;9:66.  WB ;  Rat.  
[IF=5.58] Zhang, Qian-Qian, et al. "CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment." Scientific reports 5 (2015).  IF(IHC-P) ;  Mouse.  
[IF=5.31] Meiju Zhang. et al. The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells. J Cell Mol Med. 2022 Feb;26(4):1128-1143  IHC ;  Mouse.  
[IF=5.201] Qian Liet al. LOX-1 Regulates P. gingivalis-Induced Monocyte Migration and Adhesion to Human Umbilical Vein Endothelial Cells. Front Cell Dev Biol . 2020 Jul 14;8:596.  WB ;  Human.  
[IF=4.784] Zhan Q et al. Structural Characterization and Immunomodulatory Activity of a Novel Acid Polysaccharide Isolated from the Pulp of Rosa laevigata Michx Fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9.  Other ;  Mouse.  
[IF=4.648] Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313  FCM ;  Rat.  
[IF=4.545] Zhixiong Li et al. Shuxuening injection facilitates neurofunctional recovery via down-regulation of G-CSF-mediated granulocyte adhesion and diapedesis pathway in a subacute stroke Mousemodel. Biomed Pharmacother. 2020 Jul;127:110213.  IHC ;  Mouse.  
[IF=4.486]   
[IF=4.225] Honggang Chi. et al. Shaoyao Decoction Inhibits Inflammation and Improves Intestinal Barrier Function in Mice With Dextran Sulfate Sodium-Induced Colitis. Front Pharmacol. 2021; 12: 524287  IHC ;  Mouse.  
[IF=4.178] Jiexia Wen. et al. Lnc-17Rik promotes the immunosuppressive function of Myeloid-Derived suppressive cells in esophageal cancer. CELL IMMUNOL. 2023 Mar;385:104676  IF ;  Human,Mouse.  
[IF=4.167] Yao Li. et al. Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis. J Anim Sci Biotechno. 2021 Dec;12(1):1-16  IF ;  Rat.  
[IF=3.575] Chengjie Wu. et al. A new method for primary culture of microglia in rats with spinal cord injury. Biochem Bioph Res Co. 2022 Apr;599:63  IF ;  Rat.  
[IF=3.464] Xiao Yichen. et al. Galectin-3 Is a Crucial Immunological Disease Marker in Patients with Fungal Keratitis. DIS MARKERS. 2022;2022:1380560  IF ;  Human.  
研究领域 细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human (predicted: Pig)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 125kDa
检测分子量 165-170 kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD11b: 77-180/1152 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Function:
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Subcellular Location:
Membrane.

Tissue Specificity:
Predominantly expressed in monocytes and granulocytes.

Similarity:
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6). Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

SWISS:
P05555

Gene ID:
16409

Database links:

Entrez Gene: 3684 Human

Entrez Gene: 16409 Mouse

Omim: 120980 Human

SwissProt: P11215 Human

SwissProt: P05555 Mouse

Unigene: 172631 Human

Unigene: 262106 Mouse



产品图片
Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Rat Spleen tissue lysates
Primary: Anti-CD11b (bs-1014R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 175 kDa
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD11b/Integrin αM/Integrin Alpha M Polyclonal Antibody, Unconjugated (bs-1014R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U937 (blue).
Primary Antibody:Rabbit Anti- CD11b antibody(bs-1014R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-1014R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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