| 产品编号 | bs-0642R |
| 英文名称 | MAdCAM-1 |
| 中文名称 | 粘膜血管定居因子抗体 |
| 别 名 | Mucosal addressin cellular adhesion molecule-1; Addressin mucosal; hMAdCAM 1; MACAM1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1; MADCA_RAT. |
| 研究领域 | 肿瘤 心血管 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat MAdCAM-1: 151-250/394 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] Function: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes (By similarity). Subunit: Homodimer (Probable). Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Detected in Peyer patches and mesenteric lymph nodes but not in spleen. Similarity: Contains 3 Ig-like (immunoglobulin-like) domains. SWISS: O70540 Gene ID: 54266 Database links: Entrez Gene: 17123 Mouse SwissProt: Q61826 Mouse Unigene: 391556 Mouse MAdCAM-1（粘膜血管定居因子或黏膜地址素细胞黏附分子）、CD44等均参与癌症和肿瘤细胞与血管内皮的相互作用，与癌症和肿瘤细胞一起穿过血管内皮而进入血管内，参与血行播散转移。 |
| 产品图片 |
Sample:
Lymph node (Mouse) Lysate at 30 ug Spleen (Mouse) Lysate at 30 ug Primary: Anti- MAdCAM-1 (bs-0642R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD 
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-MAdCAM-1 Polyclonal Antibody, Unconjugated(bs-0642R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
