| 产品编号 | bs-0815R |
| 英文名称 | Megsin Rabbit pAb |
| 中文名称 | 丝氨酸蛋白酶抑制剂B7抗体 |
| 别 名 | SER-PINB7; MEGSIN; Mesangium predominant gene megsin; MGC120014; MGC120015; Serpin B7; Serpin peptidase inhibitor clade B(ovalbumin) member 7; TP55; SPB7_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Megsin: 301-380/380 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Serpin B7 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in a search for agents responsible for thrombopoiesis, and maturation of megakaryocytes. SerpinB7 was identified as a protein that increased acetylcholine esterase stimulation from bone barrow progenitor cells, and injection of SerpinB7 in mice induced a 40% increase in platelets compared to controls. Shortly thereafter, in a search for kidney specific genes, SerpinB7 was described as a protein specific to mesangial cells, and was named megsin to indicate the tissue specificity of the protein. Function: Might function as an inhibitor of Lys-specific proteases. Might influence the maturation of megakaryocytes via its action as a serpin. Subcellular Location: Cytoplasm. Tissue Specificity: Predominantly expressed in mesangial cells. Similarity: Belongs to the serpin family. Ov-serpin subfamily. SWISS: O75635 Gene ID: 8710 Database links: Entrez Gene: 8710 Human Omim: 603357 Human SwissProt: O75635 Human Unigene: 138202 Human Unigene: 742388 Human megsin是新近发现的蛋白质,属于丝氨酸蛋白酶抑制剂（Serpin）超家族，特异表达于肾小球系膜细胞,其表达异常在肾脏疾病进展中具有重要作用,megsin蛋白适量的表达，对维持人体正常的生理病理过程如凝血、纤维溶解、间质代谢、发育、血压、肿瘤和炎症等是很重要的。在系膜细胞增生和系膜基质增多明显的IgA肾病肾小球中，megsin的表达显著上调。 |
| 产品图片 |
Sample:
Lane 1: Ear (Rat) Lysate at 40 ug
Lane 2: Tongue (Rat) Lysate at 40 ug
Primary: Anti-Megsin (bs-0815R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin ) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin ) Polyclonal Antibody, Unconjugated (bs-0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Megsin Polyclonal Antibody, Unconjugated(bs-0815R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
