| 产品编号 | bs-1044R |
| 英文名称 | MIF |
| 中文名称 | 巨噬细胞移动抑制因子抗体 |
| 别 名 | Macrophage Migration Inhibitory Factor; GIF; GLIF; Glycosylation inhibiting factor; MIF protein; MMIF; Phenylpyruvate tautomerase; acrophage migration inhibitory factor (glycosylation-inhibiting factor); L-dopachrome isomerase; MIF_HUMAN. |
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Specific References (3) | bs-1044R has been referenced in 3 publications.
[IF=13.2] Hiress et al. Proinflammatory Signature of the Dysfunctional Endothelium in Pulmonary Hypertension. Role of the Macrophage Migration Inhibitory Factor/CD74 Complex. (2015) Am.J.Respir.Crit.Care.Med. 192:983-97 FC/FACS ; Human.
[IF=1.663] Hou J et al. The expression of macrophage migration inhibitory factor and intercellular adhesion molecule-1 in rats with periodontitis and atherosclerosis. Arch Oral Biol. 2019 Aug 10;107:104513. IHC-P ; Rat.
[IF=1.189] Xu Q et al. Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis. Int J Ophthalmol. 2019 May 18;12(5):711-716. WB ; Rat&Human.
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| 研究领域 | 细胞生物 免疫学 信号转导 生长因子和激素 转录调节因子 淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Dog,Pig,Cow) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12kDa |
| 细胞定位 | 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MIPF: 51-115/115 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] Function: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Subunit: Homotrimer. Interacts with CXCR2 extracellular domain. Interacts with the CD74 extracellular domain, COPS5 and BNIPL. Subcellular Location: Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. DISEASE: Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. Similarity: Belongs to the MIF family. SWISS: P14174 Gene ID: 4282 Database links: Entrez Gene: 4282 Human Entrez Gene: 17319 Mouse Omim: 153620 Human SwissProt: P14174 Human SwissProt: P34884 Mouse Unigene: 407995 Human Unigene: 2326 Mouse Unigene: 2326 Mouse Unigene: 2661 Rat 生长因子和激素（ Growth Factor and Hormones） 巨噬细胞移动抑制因子是一种前炎症因子,可由多种细胞产生,它既是一种细胞因子,又是一种源于垂体的激素,还可以作为糖皮质激素生理活动的负反馈调节剂.随着巨噬细胞移动抑制因子cDNA的成功克隆及其结构的阐明,巨噬细胞移动抑制因子的特殊生物学活性及其在多种疾病中的重要作用已日益为科研人员所重视。 |
| 产品图片 |
Sample:
Lane 1: Rat Aldrenal gland Lysates Lane 2: Human HL-60 cell Lysates Primary: Anti-MIF (bs-1531R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12kDa Observed band size: 12kDa 
Tissue/cell: skin of rat foot tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MIF Polyclonal Antibody, Unconjugated(bs-1044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MIF Polyclonal Antibody, Unconjugated(bs-1044R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
