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Rabbit Anti-MMP13 antibody (bs-0575R)
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说明书: 50ul  100ul  200ul
20ul/480.00元
50ul/880.00元
100ul/1580.00元
200ul/2480.00元
大包装/询价

产品编号 bs-0575R
英文名称 MMP13
中文名称 基质金属蛋白酶13抗体
别    名 CLG 3; CLG3; Collagenase 3; Collagenase3; MMP13; MMP 13; MMP-13; Matrix Metalloproteinase 13; MMP 13; MMP13_HUMAN.   
Specific References  (4)     |     bs-0575R has been referenced in 4 publications.
[IF=3.82] Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013).  IHC-P ;  Rat.  
[IF=2.35] Zhang, Chao-ying, et al. "Hydrogen sulfide suppresses the expression of MMP-8, MMP-13, and TIMP-1 in left ventricles of rats with cardiac volume overload." Acta Pharmacologica Sinica (2013).  Rat.  
[IF=3.71] Ma, Chuan, et al. (2014) "Effects of Chronic Sleep Deprivation on the Extracellular Signal-Regulated Kinase Pathway in the Temporomandibular Joint of Rats." PLoS ONE 9(9) (2014):e107544  WB ;  Rat.  
[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.  
研究领域 肿瘤  心血管  信号转导  细胞骨架  细胞外基质  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用 WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 52kDa
细胞定位 细胞外基质 分泌型蛋白 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MMP13:201-300/471 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].

Function:
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.

Subcellular Location:
Secreted, extracellular space, extracellular matrix (Probable).

Tissue Specificity:
Seems to be specific to breast carcinomas.

DISEASE:
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.

Similarity:
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.

SWISS:
P45452

Gene ID:
4322

Database links:

Entrez Gene: 4322 Human

Entrez Gene: 17386 Mouse

Entrez Gene: 171052 Rat

Entrez Gene: 403763 Dog

Omim: 600108 Human

SwissProt: P45452 Human

SwissProt: P33435 Mouse

SwissProt: P23097 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

MMP13基质金属蛋白酶-13可降解Ⅰ、Ⅱ、Ⅲ型胶原,并对Ⅱ型胶原更有效果,主要用于骨与关节病变的研究。
产品图片
Sample:
Ovary (Rat)Lysate at 40 ug
Primary: Anti- MMP13 (bs-0575R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Ovary (Rat)Lysate at 40 ug
Primary: Anti- MMP13 (bs-0575R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- MMP13 (bs-0575R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
MG63 (Human)Lysate at 30 ug
U 2OS(Human)Lysate at 30 ug
MDA-MB-231(Human)Lysate at 30 ug
Primary: Anti- MMP13 (bs-0575R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 60 kD
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-MMP-13 Polyclonal Antibody, Unconjugated (bs-0575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(bs-0575R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat articular cartilage; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(bs-0575R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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