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Rabbit Anti-MMP2 antibody (bs-0412R)
~~~促销,代码SQ220725~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0412R
英文名称 MMP2
中文名称 基质金属蛋白酶2抗体
别    名 MMP-2; 72 kDa gelatinase; 72kD type IV collagenase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase; Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix Metalloproteinase 2; Matrix metalloproteinase II; MMP 2; MMP II; MONA; Neutrophil gelatinase; TBE 1; MMP2_HUMAN.  
Specific References  (46)     |     bs-0412R has been referenced in 46 publications.
[IF=2.303] Suihui Li. et al. Hsa_circ_0048674 facilitates hepatocellular carcinoma progression and natural killer cell exhaustion depending on the regulation of miR-223-3p/PDL1. Histol Histopathol. 2022 Feb 21;18440  WB,IHC ;  Human,Mouse.  
[IF=2.3] Wu, Kaijie, et al. "Silibinin reverses epithelial-to-mesenchymal transition in metastatic prostate cancer cells by targeting transcription factors." Oncology Reports 23.6 (2010): 1545.  WB ;  Human.  
[IF=0] Hiramoto, K., Y. Yamate, and E. F. Sato. "Long-Term Ultraviolet A Eye Irradiation Causes Retina Denaturation in Mice." Biomedicine Hub 2.1 (2017): 5-5.  WB ;  Mouse.  
[IF=3.14] Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).  WB ;  Human.  
[IF=2.766] Li et al. Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. (2016) PLoS.One. 11:e0148273  WB ;  Rat.  
[IF=4.486] Xu T et al. MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med . 2020 Jul;24(13):7127-7140.  WB ;  Human.  
[IF=5.064] Xia Liu. et al. The adipokine orosomucoid alleviates adipose tissue fibrosis via the AMPK pathway. Acta Pharmacol Sin. 2021 Apr;:1-9  WB ;  Mouse.  
[IF=3.199] Duan, Houyu. et al. KISS-1, Mediated by Promoter Methylation, Suppresses Esophageal Squamous Cell Carcinoma Metastasis via MMP2/9/MAPK Axis. Digest Dis Sci. 2022 Jan;:1-17  WB ;  Human.  
[IF=3.512] Wen Xiong. et al. Mechanisms and Molecular Targets of BuShenHuoXue Formula for Osteoarthritis. Acs Omega. 2022;7(5):4703–4713  WB ;  Rat.  
[IF=2.72] Sudeshna Nandi. et al. Anti-cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration. 2021 Nov 22  WB ;  Human.  
[IF=3.06] Wang, Huihui. et al. Anti-inflammatory and Antioxidant Effects of Pyrroloquinoline Quinone in L-NAME-Induced Preeclampsia-Like Rat Model. 2021 Sep 20  WB ;  Rat.  
[IF=6.291] Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634  WB ;  Mouse.  
[IF=7.097] Liting Cheng. et al. Bioresponsive micro-to-nano albumin-based systems for targeted drug delivery against complex fungal infections. Acta Pharm Sin B. 2021 May;:  IF ;  Mouse.  
[IF=1.841] Ozge Petek Erpolat. et al. Angiotensin-converting Enzyme Inhibitor Reduces Radiation-induced Periprosthetic Capsular Fibrosis. J Surg Res. 2021 Jul;263:167  IHC ;  Rat.  
[IF=3.337] Hongzhong Cheng. et al. Circular RNA circLPAR3 Facilitates Esophageal Squamous Cell Carcinoma Progression Through Upregulating HMGB1 via Sponging miR-375/miR-433. Oncotargets Ther. 2020; 13: 7759–7771  WB ;  Human.  
[IF=3.216] Yanhong Wang. et al. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats. Drug Des Dev Ther. 2020; 14: 4825–4834  IHC ;  Rat.  
[IF=2.511] Zhou L et al. The role of miR-21/RECK in the inhibition of osteosarcoma by curcumin. Mol Cell Probes. 2020 Feb 17:101534.  WB ;  Human.  
[IF=1.719] Saribas GS et al. Effects of uterus derived mesenchymal stem cells and their exosomes on asherman's syndrome. Acta Histochem. 2019 Nov 24:151465.  IHC-P ;  Rat.  
[IF=2.336] Han N et al. MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy (OIR) rat model via targeting VEGFA and HIF-1α. Clin Exp Pharmacol Physiol. 2019 Aug 13.  WB ;  Rat&Human.  
[IF=4.522] Chang J et al. miR‐363‐3p inhibits migration, invasion, and epithelial–mesenchymal transition by targeting NEDD9 and SOX4 in non‐small‐cell lung cancer. J Cell Physiol. 2019 Jul 22.  WB ;  Human.  
[IF=2.014] Han S et al. The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway. Biochem Cell Biol. 2019 Jan 24.  WB ;  Human.  
[IF=2.05] Neupane S et al.Focal localization of inflammatory cytokines and neurotrophins in a tongue chronic injury model. (2019) Archives of Oral Biology 99, 22–30.  IHC-P ;  Mouse.  
[IF=2.766] Yan et al. Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway. (2016) PLoS.One. 11:e0148536  WB ;  Rat.  
[IF=0] Ren et al. Interferon-γ and celecoxib inhibit lung-tumor growth through modulating M2/M1 macrophage ratio in the tumor microenvironment. (2014) Drug.Des.Devel.Ther. 8:1527-38  IHC ;  Mouse.  
[IF=0] Moriwaki et al. TRKB tyrosine kinase receptor is a potential therapeutic target for poorly differentiated oral squamous cell carcinoma. (2018) Oncotarget. 9:25225-25243  WB ;  mouse.  
[IF=5.11] Vendrov, Aleksandr E., et al. "Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice." Journal of the American Heart Association 6.11 (2017): e006775.  IHC-F ;  Mouse.  
[IF=1.5] Gao, Yuan, et al. "Effect of Telmisartan on Preventing Learning and Memory Deficits Via Peroxisome Proliferator-Activated Receptor-γ in Vascular Dementia Spontaneously Hypertensive Rats." Journal of Stroke and Cerebrovascular Diseases (2017).  IHC-P ;  Rat.  
[IF=3.73] Zhu, Hongwu, et al. "Activating Transcription Factor 4 Promotes Esophageal Squamous Cell Carcinoma Invasion and Metastasis in Mice and Is Associated with Poor Prognosis in Human Patients." PLoS one 9.7 (2014): e103882.  IHC-P ;  Human.  
[IF=2.53] Ni, Wei-Jian, et al. "Renoprotective effects of berberine through regulation of the MMPs/TIMPs system in streptozocin-induced diabetic nephropathy in rats."European Journal of Pharmacology (2015).  WB ;  Rat.  
[IF=3.457] Zhang J et al. Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation. (2019)Biomedicine & Pharmacotherapy, 109, 806–814.  IHC ;  Mouse.  
[IF=4.848] Chen Chenglong. et al. miR-135a Reduces Osteosarcoma Pulmonary Metastasis by Targeting Both BMI1 and KLF4. Front Oncol. 2021 Mar;11:930  IF ;  Human.  
[IF=3.15] Gong H et al. EZH2 inhibitors reverse resistance to gefitinib in primary EGFR wild-type lung cancer cellsBMC Cancer.2020 Dec 4;20(1):1189.  WB ;  Human.  
[IF=2.79] Qin, YuanHua, et al. "Recombinant human CXCL8 (3‐72) K11R/G31P regulates smooth muscle cell proliferation and migration through blockage of interleukin‐8 receptor." IUBMB life 65.1 (2013): 67-75.  Mouse.  
[IF=3.407] Du J et al. MAY, a novel tubulin inhibitor, induces cell apoptosis in A549 and A549/Taxol cells and inhibits epithelial-mesenchymal transition in A549/Taxol cells. Chem Biol Interact. 2020 May 25;323:109074.  WB ;  human.  
[IF=2.247] Tort S et al. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns. 2019 Dec 17. pii: S0305-4179(18)30976-8.  IHC-P ;  Rat.  
[IF=2.081] Luo Z et al. Ligustilide Inhibited Rat Vascular Smooth Muscle Cells Migration via c‐Myc/MMP2 and ROCK/JNK Signaling Pathway. J Food Sci. 2019 Nov 24.  WB ;  Rat.  
[IF=7.126] Vendrov AE et al.NOXA1-dependent NADPH oxidase regulates redox signaling and phenotype of vascular smooth muscle cell during atherogenesis.Redox Biol. (2018) Redox Biol. 21:101063.  IHF ;  Mouse.  
[IF=5.646] Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55.  FCM&IHC ;  Mouse&Human.  
[IF=2.25] Fan S et al.Carboxypeptidase E-ΔN promotes migration, invasion and epithelial-mesenchymal transition of human osteosarcoma cell lines through the Wnt/β-catenin pathway.(2018) Biochem Cell Biol.  WB ;  Human.  
[IF=2.25] Shi S et al. MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.(2018) Biochemistry and Cell Biology  WB ;  Rat &Human.  
[IF=4.12] Du et al. MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a. (2015) Sci.Rep. 5:16163  WB ;  Mouse.  
[IF=2.54] Wang, Li, et al. "Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model." Peptides (2017).  IHC-P ;  Rabbit.  
[IF=4.17] Madka, Venkateshwar, et al. "TP53 modulating agent, CP-31398 enhances antitumor effects of ODC inhibitor in mouse model of urinary bladder transitional cell carcinoma." American Journal of Cancer Research 5.10 (2015): 3030.  WB, IHC-P ;  Mouse.  
[IF=1.91] Fang, Ming, Xin-Chi Wu, and Wenlong Huang. "Raloxifene Upregulated Mesangial Cell MMP-2 Activity via ER-β Through Transcriptional Regulation." Cell Biochemistry and Biophysics (2013): 1-7.  WB ;  Mouse.  
[IF=4.3] Wu, Kaijie, et al. "PI3K/Akt to GSK3β/β-catenin signaling cascade coordinates cell colonization for bladder cancer bone metastasis through regulating ZEB1 transcription." Cellular signalling (2012).  Mouse.  
[IF=4.3] Wu, Kaijie, et al. "Silibinin inhibits β-catenin/ZEB1 signaling and suppresses bladder cancer metastasis via dual-blocking epithelial-mesenchymal transition and stemness." Cellular Signalling (2013).  WB ;  Human.  
研究领域 肿瘤  心血管  细胞生物  神经生物学  细胞骨架  细胞外基质  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Pig, Cow, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 72kDa
细胞定位 细胞核 细胞浆 细胞膜 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MMP2: 31-109/476 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Function:
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Subunit:
Interacts (via the C-terminal hemopexin-like domains-containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B.

Subcellular Location:
Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
Isoform 2: Cytoplasm. Mitochondrion.

Tissue Specificity:
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

Post-translational modifications:
Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro. The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

DISEASE:
Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.

Similarity:
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.

SWISS:
P08253

Gene ID:
4313

Database links:

Entrez Gene: 4313 Human

Entrez Gene: 17390 Mouse

Entrez Gene: 81686 Rat

Omim: 120360 Human

SwissProt: P08253 Human

SwissProt: P33434 Mouse

SwissProt: P33436 Rat

Unigene: 513617 Human

Unigene: 29564 Mouse

Unigene: 6422 Rat




合成与降解(Synthesis and Degradation) 基质金属蛋白酶(matrix metalloproteinases, MMPs)MMP2是一族依赖锌离子而降解各种细胞外基质的蛋白酶,亦称IV型胶原酶或称明胶酶A,其主要功能为降解IV型胶原,因而它在肿瘤细胞突破基底膜屏障和浸润转移中起重要作用。目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与转移浸润的研究。
产品图片
Sample:
Ovary (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-MMP2 (bs-0412R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72'62 kD
Observed band size: 62 kD
Sample:
U2os(Human) Cell Lysate at 30 ug
Primary: Anti- MMP2 (bs-0412R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 70 kD
Sample: HT1080 Cell (Human) Lysate at 40 ug
Primary: Anti-MMP2 (bs-0412R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 72 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP2) Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP2) Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Tissue/cell: rat carotid artery; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-2 Polyclonal Antibody, Unconjugated(bs-0412R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-2 Polyclonal Antibody, Unconjugated(bs-0412R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-MMP2 antibody (bs-0412R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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