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Rabbit Anti-MyD88  antibody (bs-1047R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1047R
英文名称 MyD88
中文名称 髓样分化蛋白抗体
别    名 myeloid differential protein-88; Myeloid differentiation primary response gene; MYD 88; MYD88D; Myeloid differentiation marker 88; Myeloid differentiation primary response gene 88; Myeloid differentiation primary response gene; Myeloid differentiation primary response protein MyD88; MYD 88; Myd88; MYD88_HUMAN; OTTHUMP00000161718; OTTHUMP00000208595; OTTHUMP00000209058; OTTHUMP00000209059; OTTHUMP00000209060.   
Specific References  (39)     |     bs-1047R has been referenced in 39 publications.
[IF=17.521] Yi Yan. et al. Nanomedicines Reprogram Synovial Macrophages by Scavenging Nitric Oxide and Silencing CA9 in Progressive Osteoarthritis. Advanced Science. 2023 Feb;:2207490  WB ;  Mouse.  
[IF=9.038] Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:124050  WB ;  Human.  
[IF=8.039] Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse.  
[IF=6.286] Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN-β signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044  WB ;  Chicken.  
[IF=5.988] Guirong Chen. et al. The interaction of MD-2 with small molecules in huanglian jiedu decoction play a critical role in the treatment of sepsis.. FRONT PHARMACOL. 2022 Sep;13:947095-947095  WB ;  Mouse.  
[IF=5.895] Xue-Yan Dai. et al. Role of Toll-like Receptor/MyD88 Signaling in Lycopene Alleviated Di-2-ethylhexyl Phthalate (DEHP)-Induced Inflammatory Response. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX  WB, IF ;  Mouse.  
[IF=5.875] Tengteng Yuan. et al. PF-PLC micelles ameliorate cholestatic liver injury via regulating TLR4/MyD88/NF-κB and PXR/CAR/UGT1A1 signaling pathways in EE-induced rats. Int J Pharmaceut. 2022 Mar;615:121480  WB ;  Rat.  
[IF=5.31] Xueqing Hu. et al. Tacrolimus alleviates LPS-induced AKI by inhibiting TLR4/MyD88/NF-κB signalling in mice. 2021 Dec 09  WB ;  Mouse.  
[IF=5.162] Liu S et al. Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells. Int J Biol Macromol . 2020 Oct 15;161:797-809.  WB ;  Mouse.  
[IF=5.085] Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441  
[IF=4.868] Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.  WB ;  Mouse.  
[IF=4.571] Jian-Zheng Yang. et al. Obeticholic acid protects against methamphetamine-induced anxiety-like behavior by ameliorating microbiota-mediated intestinal barrier impairment. TOXICOLOGY. 2023 Mar;486:153447  IF ;  Mouse.  
[IF=4.486] Shun Huang. et al. Design, Synthesis, and Activity Study of Cinnamic Acid Derivatives as Potent Antineuroinflammatory Agents. Acs Chem Neurosci. 2021;12(3):419–429  WB ;  Mouse.  
[IF=4.35] Yun-Jiao Shi. et al. Impact of the Plastein Reaction of Casein Hydrolysates in the Presence of Exogenous Amino Acids on Their Anti-Inflammatory Effect in the Lipopolysaccharide-Stimulated Macrophages. Foods. 2022 Jan;11(2):196  WB ;  Mouse.  
[IF=4.26] Li, Chengcheng, et al. "Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway." Scientific Reports 7 (2017).  WB ;  Mouse.  
[IF=4.23] Xiaoyin Zhang. et al. Proinflammatory S100A9 stimulates TLR4/NF-κB signaling pathways causing enhanced phagocytic capacity of microglial cells. IMMUNOL LETT. 2023 Mar;255:54  WB ;  Mouse.  
[IF=4.162] Jiali Yang. et al. Baicalin Rescues Cognitive Dysfunction, Mitigates Neurodegeneration, and Exerts Anti-Epileptic Effects Through Activating TLR4/MYD88/Caspase-3 Pathway in Rats. Drug Des Dev Ther. 2021 Jul;15:3163-3180  IHC ;  Rat.  
[IF=3.913] Canan Eroğlu Güneş. et al. Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells. GENE. 2022 Aug;:146805  WB ;  Human.  
[IF=3.889] Ming Dong Si. et al. Swertia mussotii prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-κB. PHARM BIOL. 2022 Oct 07  WB ;  Rat.  
[IF=3.88] Fu, Juanli, et al. "Tetrachlorobenzoquinone exerts neurological pro-inflammatory activity by promoting HMGB1 release, which induces TLR4 clustering within the lipid raft." Toxicological Sciences (2016): kfw124.  WB, IP ;  Rat.  
[IF=3.82] Fu, Juanli, et al. "The acute exposure of tetrachloro-p-benzoquinone (aka chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning." Toxicology (2017).  WB ;  Rat.  
[IF=3.69]   WB ;  rat.  
[IF=3.69] Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-κB and TGF-β/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275  WB ;  Rat.  
[IF=3.524] Rossetto Isabela Maria Urra. et al. Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum. Toxicol Res-Uk. 2021 Aug;10(4):835-849  WB ;  Rat.  
[IF=3.414] Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.  WB ;  Human.  
[IF=3.26] Chen, Gangling, et al. "Limb Remote Ischemic Postconditioning Reduces Ischemia-Reperfusion Injury by Inhibiting NADPH Oxidase Activation and MyD88-TRAF6-P38MAP-Kinase Pathway of Neutrophils." International Journal of Molecular Sciences 17.12 (2016): 1971.  WB ;  Rat.  
[IF=3] Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923.  WB ;  Chicken.  
[IF=2.967] Lili Li. et al. Protein overexpression of toll‑like receptor 4 and myeloid differentiation factor 88 in oral squamous cell carcinoma and clinical significance. Oncol Lett. 2021 Nov;22(5):1-11  IHC ;  human.  
[IF=2.952] Yongfeng Zhang. et al. Protection against ulcerative colitis and colorectal cancer by evodiamine via anti‑inflammatory effects. Mol Med Rep. 2022 May;25(5):1-14  WB ;  Mouse.  
[IF=2.717] Xu D et al. Saturated fatty acids activate the inflammatory signalling pathway in Schwann cells: Implication in sciatic nerve injury. Scand J Immunol . 2020 Aug;92(2):e12896.  WB ;  Rat.  
研究领域 细胞生物  免疫学  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human
产品应用 WB=1:200-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 34kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse MyD88: 201-296/296 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010].

Function:
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.

Subunit:
Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1 (By similarity). Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. May interact with PIK3AP1 (By similarity). Upon IL1B treatment, forms a complex with PELI1, IRAK1, IRAK4 and TRAF6; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitous.

DISEASE:
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.

Similarity:
Contains 1 death domain.
Contains 1 TIR domain.

SWISS:
P22366

Gene ID:
17874

Database links:

Entrez Gene: 4615 Human

Entrez Gene: 17874 Mouse

Entrez Gene: 301059 Rat

Omim: 602170 Human

SwissProt: Q99836 Human

SwissProt: P22366 Mouse

SwissProt: Q6Y1S1 Rat

Unigene: 82116 Human

Unigene: 213003 Mouse

Unigene: 37341 Rat



MyD88是天然免疫中的调控分子,可能在感染、炎症、免疫等病理生理过程中具有更广泛的生物学功能, MyD88蛋白是由Toll样受体介导的先天免疫应答反应中重要的胞浆接头蛋白,由它参与构成的信号级联最终引起NF-kB依赖性信号通路的活化。
产品图片
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Lymph node tissue lysates
Primary: Anti-MyD88 (bs-1047R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 30 kDa
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti- MyD88 (bs-1047R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 32 kD
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MyD88 Polyclonal Antibody, Unconjugated(bs-1047R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MyD88) Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MyD88) Polyclonal Antibody, Unconjugated (bs-1047R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HO-1) Polyclonal Antibody, Unconjugated (bs-0827R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-MyD88 antibody (bs-1047R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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