| 产品编号 | bs-0337R |
| 英文名称 | Myelin Protein Zero |
| 中文名称 | 外周髓磷脂P0蛋白/P0蛋白抗体 |
| 别 名 | peripheral myelin prothein Zero; MPZ; MPP; Myelin P0 protein; CHM; CMT1; CMT1B; DSS; MYELIN GLYCOPROTEIN P-ZERO; MYELIN PROTEIN PERIPHERAL; Myelin protein zero; P0; MYP0_HUMAN. |
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Specific References (6) | bs-0337R has been referenced in 6 publications.
[IF=10.76] Schulz, Alexander, et al. "The importance of nerve microenvironment for schwannoma development." Acta Neuropathologica (2016): 1-19. IHC-P ; Mouse.
[IF=9.91] Schulz, Alexander, et al. ʺNeuronal merlin influences ERBB2 receptor expression on Schwann cells through neuregulin 1 type III signalling.ʺ Brain (2013): awt327 IHC-P ; Human.
[IF=6] Röhr, Dominik, et al. "Sodium‐dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation." Glia (2017). WB, IF(ICC) ; Mouse.
[IF=4.73] Erdal Ayhan Işik. et al. Use of Erythropoietin and Fibrin Glue Mixture for Peripheral Nerve Repair. Plast Reconstr Surg. 2022 Feb;149(2):395-403 IHC ; Rat.
[IF=3.73] Li, Junhui, et al. "Relationship between neural alteration and perineural invasion in pancreatic cancer patients with hyperglycemia." PLoS One 6.2 (2011): e17385. IHC-P ; Human.
[IF=2.27] Chitose, Shun‐ichi, et al. "Recurrent laryngeal nerve regeneration using an oriented collagen scaffold containing Schwann cells." The Laryngoscope (2016). IHC-P ; Dog.
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| 研究领域 | 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Dog,Rat,Mouse (predicted: GuineaPig,Rabbit,Horse,Cow,Pig,Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 24kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P0 protein: 175-248/248 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Myelin protein-zero is the major structural protein of peripheral myelin, accounting for more than 50% of the protein present in the sheath of peripheral nerves. Expression of the gene encoding Myelin protein-zero (MPZ) is restricted to Schwann cells; MPZ is not found in the CNS. An integral membrane glycoprotein of ~28kDa, Myelin protein-zero is thought to link adjacent lamellae and thereby stabilize the myelin assembly. The other 3 major components of myelin are myelin basic protein, myelin proteolipid protein and myelin-associated glycoprotein. Subunit: Homodimer and homotetramer. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Found only in peripheral nervous system Schwann cells. Similarity: Belongs to the myelin P0 protein family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P25189 Gene ID: 4359 Database links: Entrez Gene: 4359 Human Entrez Gene: 17528 Mouse Omim: 159440 Human SwissProt: P25189 Human SwissProt: P27573 Mouse Unigene: 591486 Human Unigene: 93883 Human Unigene: 9986 Mouse UniProtKB/Swiss-Prot: P25189.1
成熟的周围神经系统髓鞘主要的蛋白为P0蛋白，分子量28KDa，它具有单个跨膜区，是IgG超家族的成员之一。 |
| 产品图片 |
Sample:
Spinal cord (Rat) Lysate at 40 ug Primary: Anti- Myelin Protein Zero (bs-0337R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 29 kD 
Sample:
Bone (Mouse) Lysate at 40 ug Primary: Anti-Myelin Protein Zero (Bs- 0337R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kD 
Sample:
Lane 1: Spinal cord (Mouse) Lysate at 40 ug Lane 2: Spinal cord (Rat) Lysate at 40 ug Lane 3: Adipose (Mouse) Lysate at 40 ug Lane 4: Adipose (Rat) Lysate at 40 ug Primary: Anti-Myelin Protein Zero (bs-0337R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
