| 产品编号 | bs-0073R |
| 英文名称 | Neurturin Rabbit pAb |
| 中文名称 | 神经营养因子抗体 |
| 别 名 | Neurturin precursor; Neurturin; NRTN; NTN; NRTN_HUMAN. |
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Specific References (2) | bs-0073R has been referenced in 2 publications.
[IF=6.1] Jing Man. et al. TANGO1 interacts with NRTN to promote hepatocellular carcinoma progression by regulating the PI3K/AKT/mTOR signaling pathway. BIOCHEM PHARMACOL. 2023 Jul;213:115615 ICC,WB ; Human.
[IF=2.46] Lu, Yanmei, et al. "Effect of Low-level Vagus Nerve Stimulation on Cardiac Remodeling in a Rapid Atrial Pacing–induced Canine Model of Atrial Fibrillation." Journal of cardiovascular pharmacology 67.3 (2016): 218-224. IHC-P ; Dog.
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| 研究领域 | 发育生物学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Neurturin: 151-197/197 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Human Neurturin (NTN) and Human GDNF comprise a family of TGF-beta related neurotrophic factor which have trophic influences on a variety of neuronal populations. Neurturin promotes the survival of certain sympathetic and sensory neurins through interaction with distinct set of GDNF-like receptors
Function: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted. DISEASE: Note=Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Similarity: Belongs to the TGF-beta family. GDNF subfamily. SWISS: Q99748 Gene ID: 4902 Database links: Entrez Gene: 4902 Human Entrez Gene: 18188 Mouse Omim: 602018 Human SwissProt: Q99748 Human SwissProt: P97463 Mouse Unigene: 234775 Human Unigene: 8074 Mouse Unigene: 21952 Rat |
| 产品图片 |
Sample:
Lane 1: Human K562 cell Lysates
Lane 2: Human 293T cell Lysates
Primary: Anti-Neurturin (bs-0073R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21kDa
Observed band size: 21kDa
Sample:
Placenta(Mouse)lysate at 30 ug;
Brain(Mouse) lysate at 30 ug;
Primary: Anti- Neurturin (bs-0073R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 21 kD
Observed band size: 21 kD
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Neurturin Polyclonal Antibody, Unconjugated(bs-0073R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
