| 产品编号 | bs-0178R |
| 英文名称 | Nurr1 |
| 中文名称 | 核受体相关因子-1抗体 |
| 别 名 | Nur-ralated factor 1; Nuclear receptor-related factor 1; Orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; Regenerating liver nuclear receptor 1; RNR-1; SL-322; Nuclear orphan receptor HZF-3; T-cell nuclear receptor NOT;HZF 3; HZF3; Immediate-early response protein NOT; Intermediate early receptor protein; NGFI B/nur77 beta type transcription factor homolog; NOT; NR4A2; NR4A2_HUMAN; Nuclear receptor subfamily 4 group A member 2; Nur related protein 1 homolog; Nurr 1;RNR 1; RNR1; TINUR; Transcriptionally inducible nuclear receptor; Transcriptionally inducible nuclear receptor related; Transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor. |
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Specific References (1) | bs-0178R has been referenced in 1 publications.
[IF=2.447] Qiu Qian. et al. Propofol protects H9C2 cells against hypoxia/reoxygenation injury through miR‑449a and NR4A2. Exp Ther Med. 2021 Oct;22(4):1-11 WB ; Rat.
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| 研究领域 | 染色质和核信号 神经生物学 新陈代谢 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 67kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nurr1: 301-400/598 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Nuclear receptor and transcription factor; plays a role in development and maintenance of neurons synthesizing the neurotransmitter dopamine This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full length nature has not been determined. Function: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Subunit: Interacts with SFPQ, NCOR2, SIN3A and HADC1. The interaction with NCOR2 increases in the absence of PITX3 (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. Similarity: Belongs to the nuclear hormone receptor family. NR4 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P43354 Gene ID: 4929 Database links: Entrez Gene: 4929 Human Entrez Gene: 18227 Mouse Omim: 601828 Human SwissProt: P43354 Human SwissProt: Q06219 Mouse Unigene: 563344 Human Unigene: 3507 Mouse Unigene: 88129 Rat 该抗体和类固醇/甲状腺素受体密切相关的早期应急反应基因，也受各种生长因子的诱导。中脑多巴胺神经细胞和脑干细胞表达。 |
| 产品图片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: U87MG (Human) Cell Lysate at 30 ug Lane 3: MCF-7 (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Lane 5: Raii (Human) Cell Lysate at 30 ug Lane 6: U251 (Human) Cell Lysate at 30 ug Primary: Anti-Nurr1 (bs-0178R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD 
Sample: Raji Cell (Human) Lysate at 40 ug
Primary: Anti- Nurr1 (bs-0178R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 65 kD 
Sample:
MCF-7 Cell (Human) Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti- Nurr1 (bs-0178R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 70 kD 
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nurr1) Polyclonal Antibody, Unconjugated (bs-0178R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
