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Rabbit Anti-OPG antibody (bs-0431R)
~~~促销,代码SSY221101~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0431R
英文名称 OPG
中文名称 骨保护蛋白/护骨素抗体
别    名 MGC29565; OCIF; OPG; Osteoclastogenesis Inhibitory Factor; TNFRSF 11B; TNFRSF11B; TR 1; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; TR11B_HUMAN.  
Specific References  (31)     |     bs-0431R has been referenced in 31 publications.
[IF=6.291] Ruijun Su. et al. Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE-/- mice. Ecotox Environ Safe. 2022 Apr;234:113410  WB,IHC ;  Mouse,Human.  
[IF=6.289] Yutao Cui. et al. Functionalized anti-osteoporosis drug delivery system enhances osseointegration of an inorganic–organic bioactive interface in osteoporotic microenvironment. Mater Design. 2021 Aug;206:109753  IHC ;  Rabbit.  
[IF=3.24] Hiroki Ehara. et al. Role of tissue factor in delayed bone repair induced by diabetic state in mice. Plos One. 2021 Dec;16(12):e0260754  WB ;  Mouse.  
[IF=1.813] Wang Tong. et al. Acupotomy Contributes to Suppressing Subchondral Bone Resorption in KOA Rabbits by Regulating the OPG/RANKL Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8168657  WB,IHC ;  Rabbit.  
[IF=3.998] Daisuke Nishida. et al. RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp. Sci Rep-Uk. 2021 Feb;11(1):1-12  IHC ;  Mouse.  
[IF=3.998] M M et al. Pantoprazole impairs fracture healing in aged miceSci Rep.2020 Dec 23;10(1):22376.  WB ;  Mouse.  
[IF=2.304] Teng X et al. Effects of Low Dietary Phosphorus on Tibia Quality and Metabolism in Caged Laying Hens. Prev Vet Med . 2020 Aug;181:105049.  WB ;  Hen.  
[IF=2.31] Wei B et al. Osteoprotegerin/bone morphogenetic protein 2 combining with collagen sponges on tendon-bone healing in rabbits. J Bone Miner Metab. 2020 Jan 24.  ELISA ;  Rabbit.  
[IF=2.048] Yu X et al. Substance P aggravates periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019.  WB ;  Rat&Mouse.  
[IF=2.048] Yan KX et al. Substance P participates in periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019 Dec.  WB ;  Rat.  
[IF=6.383] Orth M et al. Effects of locally applied adipose tissue-derived microvascular fragments by thermoresponsive hydrogel on bone healing.Acta Biomater. 2018 Sep 1;77:201-211.  WB ;  Mouse.  
[IF=2.412] Yu X et al. CGRP gene-modified rBMSCs show better osteogenic differentiation capacity in vitro.J Mol Histol. 2018 Aug;49(4):357-367.  WB ;  Rat.  
[IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).  WB ;  Human.  
[IF=1.75] Chen, Helin, et al. "Intermittent administration of parathyroid hormone ameliorated alveolar bone loss in experimental periodontitis in streptozotocin-induced diabetic rats." Archives of Oral Biology (2017).  IHC-P ;  Rat.  
[IF=1.6] He, Ming, et al. "Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis." Molecular medicine reports 14.2 (2016): 1054-1060.  Mouse.  
[IF=2.62] Zhang, Xiaonan, et al. "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway."Chemico-Biological Interactions (2016).  WB ;  Rat.  
[IF=2.68] Xiao, Wanan, et al. "Bone fracture healing is delayed in splenectomic rats." Life Sciences (2016).  WB ;  Rat.  
[IF=2.09] Chen, Zhiguang, et al. "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway."International Journal of Molecular Medicine (2016).  WB ;  Rat.  
[IF=2.99] Herath, Steven C., et al. "Stimulation of angiogenesis by cilostazol accelerates fracture healing in mice." Journal of Orthopaedic Research (2015).  WB ;  Mouse.  
[IF=1.98] Yu, Xijiao, et al. "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." Journal of Molecular Histology: 1-9.  WB ;  Rat.  
[IF=1.27] Zhang, Yuanyu, et al. "Mycobacterium tuberculosis 10-kDa co-chaperonin regulates the expression levels of receptor activator of nuclear factor-κB ligand and osteoprotegerin in human osteoblasts." Experimental and therapeutic medicine 9.3 (2015): 919-924.  WB ;  Human.  
[IF=1.88] Li, Xianxian, et al. "Oral administration of 5-Hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats." Archives of Oral Biology(2015).  IHC-P ;  Rat.  
[IF=13.273] Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094  WB ;  Mouse.  
[IF=3.701] Shan Shan Qi. et al. Lycopene ameliorates diabetic osteoporosis via anti-inflammatory, anti-oxidation, and increasing Osteoprotegerin/RANKL expression ratio. J Funct Foods. 2021 Aug;83:104539  IHC ;  Rat.  
[IF=2.082] Baixiang Wang. et al. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro. J Zhejiang Univ-Sc B. 2021 May;22(5):410-420  WB ;  Mouse.  
[IF=3.297] Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127  WB ;  Rat.  
[IF=4.225] Yang Y et al. Ganoderma lucidum Immune Modulator Protein rLZ-8 Could Prevent and Reverse Bone Loss in Glucocorticoids-Induced Osteoporosis Rat Model. Front Pharmacol. 2020 May 19;11:731.  WB ;  Rat.  
[IF=0.444] Ding M et al. Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats. Tropical Journal of Pharmaceutical Research. March 2019; 18 (3): 563-570.  Rat.  
[IF=2.959] Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546  WB ;  Human.  
[IF=2.66] Yu, X., et al. "Denervation effectively aggravates rat experimental periodontitis." Journal of Periodontal Research (2017).  WB ;  Rat.  
[IF=1.72] Li, Ping. "Efficacy and Safety of Echinacoside in a Rat Osteopenia Model." Evidence-Based Complementary and Alternative Medicine 2013 (2013).  Rat.  
研究领域 肿瘤  细胞生物  免疫学  信号转导  骨髓细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Cow, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 43kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human OPG: 221-300/401 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. OPG acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis with increased osteoclast activity. OPG consists of 401 amino acids with a molecular weight of 44 kDa as a monomer and 90 kDa as a disulphide-linked dimer.

Function:
Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

Subunit:
Homodimer.

Subcellular Location:
Secreted.

Tissue Specificity:
Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

Post-translational modifications:
N-glycosylated. Contains sialic acid residues.
The N-terminus is blocked.

DISEASE:
Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.

Similarity:
Contains 2 death domains.
Contains 4 TNFR-Cys repeats.

SWISS:
O08712

Gene ID:
4982

Database links:

Entrez Gene: 4982 Human

Omim: 602643 Human

SwissProt: O00300 Human

Unigene: 81791 Human



护骨素又称护骨因子(osteorotegenin,OPG)、骨保护蛋白、破骨细胞生成抑制因子、骨保护素、破骨细胞抑制生成因子(osteoclastogenesis inhibitory factor,OCIF)
OPG属于TNF受体超家族,主要功能是抑制破骨细胞的分化、成熟破骨细胞的骨吸收活性,并诱导其凋亡。
产品图片
Sample:
Heart (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-OPG(bs-0431R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43kD
Observed band size: 50kD
Sample:
Lane 1: Raji (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-OPG (bs-0431R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-OPG (bs- 0431R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 60 kD
Sample:
Placenta (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-OPG (bs- 0431R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 60 kD
Tissue/cell: rat tibia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Osteoprotegerin Polyclonal Antibody, Unconjugated(bs-0431R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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