| 产品编号 | bs-0019R |
| 英文名称 | Osteopontin |
| 中文名称 | 骨桥蛋白/分泌型磷蛋白1抗体 |
| 别 名 | BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin. |
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Specific References (6) | bs-0019R has been referenced in 6 publications.
[IF=3.539] Shunli Feng. et al. SPP1 as a key gene in the lymph node metastasis and a potential predictor of poor prognosis in head and neck carcinoma. J ORAL PATHOL MED. 2022 Jul 13 WB, IHC ; Human.
[IF=3.23] Liu C, Zhang Y, Wang L, Zhang X, Chen Q, Wu B (2015) A Strontium-Modified Titanium Surface Produced by a New Method and Its Biocompatibility In Vitro. PLoS ONE 10(11): e0140669. WB ; Rat.
[IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017). WB ; Human.
[IF=2.766] Li et al. Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. (2016) PLoS.One. 11:e0148273 IF ; Rat.
[IF=2.538] Xinxin Yu. et al. OPN up-regulated proliferation and invasion of HNSCC through p38MAPK signaling pathway. OR SURG OR MED OR PA. 2023 Mar;: WB,IHC ; Human.
[IF=0] Wei, Min Jun, et al. "Identification of OPN, TNC and E-selectin as potential recognition proteins in cerebral vasospasm after subarachnoid hemorrhage."Chinese Neurosurgical Journal 2.1 (2016): 1. WB, IHC-P ; Rat.
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| 研究领域 | 免疫学 神经生物学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Sheep,Cow,Pig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OPN: 141-220/314 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.
Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma. Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity). Subunit: Ligand for integrin alpha-V/beta-3. Subcellular Location: Secreted. Tissue Specificity: Bone. Found in plasma. Similarity: Belongs to the osteopontin family. SWISS: P10451 Gene ID: 6696 Database links: Entrez Gene: 6696 Human Entrez Gene: 20750 Mouse Omim: 166490 Human SwissProt: P10451 Human SwissProt: P10923 Mouse Unigene: 313 Human Unigene: 288474 Mouse Unigene: 8871 Rat 信号传导（Signaling Intermediates） 骨桥蛋白(osteopontin，OPN)是一种含精氨酸—甘氨酸—天冬氨酸(Arg-Gly-Asp，RGD)的分泌型糖基化磷蛋白，已归类于细胞外基质(extracellular matrix，ECM)蛋白。 OPN既是细胞因子，又是细胞黏附分子.又称：分泌性磷酸化蛋白质1 OPN最初是从骨组织分离的，而后在上皮细胞、胃肠道的分泌物、肾、甲状腺、睾丸以及一些肿瘤细胞里都陆续的发现了OPN的表达。在生殖方面，OPN在分泌期子宫内膜上皮细胞、侵袭的滋养细胞、蜕膜细胞中表达。 |
| 产品图片 |
Sample: A549 Cell Lysate at 40 ug
Primary: Anti-ostepontin (bs-0019R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 50 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
