| 产品编号 | bs-0723R |
| 英文名称 | P63 Rabbit pAb |
| 中文名称 | 肿瘤抑制基因p63抗体 |
| 别 名 | P63_HUMAN; Tumor protein 63; TP63; Chronic ulcerative stomatitis protein(CUSP); Keratinocyte transcription factor KET; Transformation-related protein 63(TP63); Tumor protein p73-like(p73L); p40; p51; KET; P63; P73H; P73L; TP73L; AIS; LMS; NBP; RHS; p40; p51; EEC3; OFC8; SHFM4; TP53L; p53CP; TP53CP; B(p51A); B(p51B); P51A; TAp63gamma; P51B; TAp63alpha; P51delta; TAp63delta; P51delNalpha; deltaNp63 alpha; P51delNbeta; deltaNp63 beta; P51delNgamma; deltaNp63gamma; p40-deltaNp63; p40-deltaNp63; |
 |
Specific References (6) | bs-0723R has been referenced in 6 publications.
[IF=5.116] Jian Liu. et al. Involvement of miRNA203 in the proliferation of epidermal stem cells during the process of DM chronic wound healing through Wnt signal pathways. Stem Cell Res Ther. 2020 Dec;11(1):1-10 IHC ; Rat.
[IF=4.197] Jianmin Li. et al. miR-184 targets TP63 to block idiopathic pulmonary fibrosis by inhibiting proliferation and epithelial–mesenchymal transition of airway epithelial cells. Lab Invest. 2020 Sep;101(2):142-154 WB,IF,IHC ; Human.
[IF=4.147] Bolun Cheng. et al. Genetic association scan of 32 osteoarthritis susceptibility genes identified TP63 associated with an endemic osteoarthritis, Kashin-Beck disease. Bone. 2021 Sep;150:115997 IHC ; Human.
[IF=2.894] TingLu Ye. et al. 5-aminolevulinic acid photodynamic therapy inhibits invasion and metastasis of SCL-1 cells probably via MTSS1 and p63 gene related pathways. Photodiagn Photodyn. 2020 Dec;32:102039 FC ; Human.
[IF=2.24] Yang Rong-Hua, et al. An improved method for the isolation and culture of rat epidermal stem cells. International Journal of Clinical and?Experimental Pathology6.11 (2013): 2529-2534. ICC ; Rat.
[IF=1.889] Shian Liao. et al. Construction of autophagy prognostic signature and analysis of prospective molecular mechanisms in skin cutaneous melanoma patients. Medicine. 2021 Jun 4; 100(22): e26219 IHC ; Human.
|
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 77 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P63 protein: 361-450/680 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq, Jul 2008]. Function: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Subunit: Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Post-translational modifications: May be sumoylated. Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. DISEASE: Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting. Similarity: Belongs to the p53 family. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q9H3D4 Gene ID: 8626 Database links: Entrez Gene: 8626 Human Entrez Gene: 22061 Mouse Omim: 603273 Human SwissProt: Q9H3D4 Human SwissProt: O88898 Mouse Unigene: 137569 Human Unigene: 20894 Mouse Unigene: 42907 Rat P63—也是肿瘤抑制因子之一.p63基因是抑癌基因p53的同源性基因，主要表达上皮组织的基底细胞或复层上皮细胞，如皮肤、食道、宫颈外口、扁桃体、膀胱等，和前列腺、乳腺腺样结构的基层细胞。 |
| 产品图片 |
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-P63 (bs-0723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 62 kD
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P63 protein/P51A Polyclonal Antibody, Unconjugated(bs-0723R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-P63（TP63） antibody (bs-0723R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
