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Rabbit Anti-PI 3 Kinase p85 alpha antibody (bs-0128R)
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说明书: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-0128R
英文名称 PI 3 Kinase p85 alpha
中文名称 磷脂酰肌醇激酶抗体
别    名 PI3-kinase p85 subunit alpha; PI3K p85; GRB1; p50 alpha; p55 alpha; p85 alpha; p85; Phosphatidylinositol 3 kinase associated p85 alpha; Phosphatidylinositol 3 kinase regulatory 1; Phosphatidylinositol 3 kinase regulatory alpha subunit; Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha); PI3 kinase p85 alpha subunit; PI3 kinase p85 subunit alpha; PI3K; PIK3R1; PtdIns 3 kinase p85 alpha; SH3_PI3K_p85alpha; P85A_HUMAN; PI 3-kinase p85α; PI 3-kinase p85 α; PI 3-kinase p85-α.  
Specific References  (16)     |     bs-0128R has been referenced in 16 publications.
[IF=1.28] Yang et al. Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. (2014) Exp.Ther.Me. 8:563-566  WB ;  Human.  
[IF=2.342] Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. Medchemcomm. 2018 Jul 19;9(9):1502-1510.  IHC-P ;  Mouse.  
[IF=2.018] Yan X et al. Antidiabetic potential of green seaweed Enteromorpha prolifera flavonoids regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice. (2018) J Food Sci.   WB ;  Mouse.  
[IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.  WB ;  Mouse.  
[IF=5.614] Zhu J et al. SPARC Promotes Self‐Renewal of Limbal Epithelial Stem Cells and Ocular Surface Restoration through JNK and p38‐MAPK Signaling Pathways. Stem Cells. 2019 Oct 23.  WB ;  Rabbit.  
[IF=3.06] Bai, Xue, et al. "Effects of water extract and crude polysaccharides from Liriope spicata var. prolifera on InsR/IRS-1/PI3K pathway and glucose metabolism in mice." Journal of ethnopharmacology 125.3 (2009): 482-486.  IHC-P ;  Mouse.  
[IF=2.94] Tian, L-Y., et al. "Anti-diabetic effect of methylswertianin and bellidifolin from Swertia punicea Hemsl. and its potential mechanism." Phytomedicine 17.7 (2010): 533-539.  IHC-P, WB ;  Mouse.  
[IF=3.33] Xu, Hong-Wu, et al. "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." BMC cancer 13.1 (2013): 247.  IHC-P ;  Human.  
[IF=1.84] Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).  WB ;  
[IF=1.28] An, Xinjiang, et al. "Role of the PTEN/PI3K/VEGF pathway in the development of Kawasaki disease." Experimental and therapeutic medicine11.4 (2016): 1318-1322.  IHC-P ;  Rabbit.  
[IF=1.69] Wang, Fan, et al. "Defective insulin signaling and the protective effects of dimethyldiguanide during follicular development in the ovaries of polycystic ovary syndrome." Molecular Medicine Reports (2017).  IHC-P ;  Rat.  
[IF=2.394] Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.  IHC-P ;  Mouse.  
[IF=2.56] Xu, C., et al. "Proteomics Analysis of Hepatocyte Proliferation Regulated by FGF, PDGF, Insulin, Oncostatin M and Interleukin 2 Signaling Pathways during Rat Liver Regeneration." J Proteomics Computational Biol 1.1 (2014): 8.  WB ;  Rat.  
[IF=3.361] Jiang A et al. Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier. Int Immunopharmacol. 2019 Nov 8:105972.  WB ;  Mouse.  
[IF=2.342] Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. MedChemComm. 2018.  IHC-P ;  Mouse.  
[IF=4.011] Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964.   WB ;  Human.  
研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Cow, Horse, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 80kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PI3 kinase p85 subunit alpha:501-600/724 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Function:
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Subunit:
Heterodimer of a regulatory subunit PIK3R1 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with FER. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Interacts with PTK2/FAK1 (By similarity). Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with LYN (via SH3 domain); this enhances enzyme activity. Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C-terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1 and phosphorylated IRS4, as well as with NISCH and HCST. Interacts with FASLG, KIT and BCR. Interacts with AXL, FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with FGR and HCK. Interacts with PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with ERBB4 (phosphorylated). Interacts with NTRK1 (phosphorylated upon ligand-binding).

Tissue Specificity:
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Post-translational modifications:
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

Similarity:
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.

SWISS:
P27986

Gene ID:
5295

Database links:

Entrez Gene: 5295 Human

Entrez Gene: 18708 Mouse

Entrez Gene: 25513 Rat

Omim: 171833 Human

SwissProt: P27986 Human

SwissProt: P26450 Mouse

SwissProt: Q63787 Rat

Unigene: 132225 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

激酶和磷酸酶(Kinases and Phosphatases)
产品图片
Sample: Heart (mouse) Lysate at 40 ug
Primary: Anti- PI3K p85 (bs-0128R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80kD
Observed band size: 85 kD
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Images provided the Independent Validation Program (badge number 029650)Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-PI3 kinase p85 alpha subunit Polyclonal Antibody (bs-0128R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated(bs-0128R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PI 3 Kinase p85 alpha) polyclonal Antibody, Unconjugated (bs-0128R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PI3K p85) polyclonal Antibody, Unconjugated (bs-0128R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Positive control: H9C2(2% Paraformaldehyde-fixed )
Isotype Control Antibody
Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier catalog number: bs-1297R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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