[IF=4.36] Xiao Li. et al. Mechanism by which Huoxue Tongluo Qiwei Decoction improves the erectile function of rats with diabetic erectile dysfunction. J Ethnopharmacol. 2022 Jan;283:114674  WB ;  Rat.  

PubMed:10.1016/j.jep.2021.114674

[IF=3.69] Ji-Sheng Wang. et al. Effect of leech-centipede medicine on improving erectile function in DIED rats via PKC signalling pathway-related molecules. J Ethnopharmacol. 2021 Mar;267:113463  WB ;  Rat.  

PubMed:33049347

Sample:
spleen (Mouse) Lysate at 40 ug
Primary: Anti-PKC beta 1 + 2 (bs-0267R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kD
Observed band size: 74 kD

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- PKC beta-1+2 Polyclonal Antibody, Unconjugated(bs-0267R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-PKC beta 1 + 2 antibody (bs-0267R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-PKC beta 1 + 2 antibody (bs-0267R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.