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Rabbit Anti-Urokinase antibody (bs-1098R)
~~~促销,代码KT001~~~
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说明书: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-1098R
英文名称 Urokinase
中文名称 尿激酶型纤溶酶原激活因子抗体
别    名 Urokinase; ATF; ATF uPA; PLAU; UPA; Plasminogen activator; Plasminogen activator urinary; Plasminogen activator urokinase; u PA; U plasminogen activator; UPA; URK; Urokinase plasminogen activator; Urokinase type plasminogen activator; Urokinase type plasminogen activator precursor.   
Specific References  (1)     |     bs-1098R has been referenced in 1 publications.
[IF=0] Liu XP et al. A Label-free Photoelectrochemical Biosensor for Urokinase-type Plasminogen Activator Detection Based on a gC 3 N 4/CdS Nanocomposite.Analytica Chimica Acta.1025;99-107.  Photoelectrochemical detection ;  
研究领域 细胞生物  免疫学  神经生物学  激酶和磷酸酶  通道蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse, Rat,  (predicted: Human, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 49kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PLAU:361-432/432 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Urokinase is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in the Urokinase gene may be associated with late onset Alzheimer disease and also with decreased affinity for fibrin binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg Val bond in plasminogen. The proprotein is cleaved at a Lys Ile bond by plasmin to form a two chain derivative in which a single disulfide bond connects the amino terminal A chain to the catalytically active, carboxy terminal B chain. This two chain derivative is also called HMW uPA (high molecular weight uPA). HMW uPA can be further processed into LMW uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino terminal fragment. LMW uPA is proteolytically active but does not bind to the uPA receptor.

Function:
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed in the prostate gland and prostate cancers.

Post-translational modifications:
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.

Similarity:
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.

SWISS:
P00749

Gene ID:
5328

Database links:

Entrez Gene: 5328 Human

Entrez Gene: 18792 Mouse

Entrez Gene: 25619 Rat

Omim: 191840 Human

SwissProt: P00749 Human

SwissProt: P06869 Mouse

SwissProt: P29598 Rat

Unigene: 77274 Human

Unigene: 4183 Mouse

Unigene: 6064 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

尿激酶型纤溶酶激活因子;是一种多效细胞因子,参与多种病理生理过程;uPA系统可通过调节纤溶酶的活性降解p淀粉样蛋白;是一种丝氨酸蛋白酶,将血浆酶原转化成血浆酶(纤维蛋白溶酶)。
产品图片
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Spleen (Mouse) Lysate at 40 ug
Lane 5: Liver (Rat) Lysate at 40 ug
Lane 6: Lung (Rat) Lysate at 40 ug
Lane 7: Spleen (Rat) Lysate at 40 ug
Primary: Anti-Urokinase (bs-1098R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40-80 kD
Observed band size: 45 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Urokinase (bs-1098R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Cerebrum (mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Urokinase (bs-1098R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49kD
Observed band size: 48kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PLAU/UPA/Urokinase Polyclonal Antibody, Unconjugated(bs-1098R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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