| 产品编号 | bs-0088R |
| 英文名称 | Secretin |
| 中文名称 | 分泌素抗体 |
| 别 名 | SCT; Secretin precursor; SECR_HUMAN; Secr. |
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Specific References (5) | bs-0088R has been referenced in 5 publications.
[IF=14.957] Yuchu Liu. et al. A gut-brain axis mediates sodium appetite via gastrointestinal peptide regulation on a medulla-hypothalamic circuit. SCI ADV. 2023 Feb;9(7) IF ; Mouse.
[IF=14.679] Lixian Chen. et al. Inhibition of secretin/secretin receptor axis ameliorates non‐alcoholic fatty liver disease phenotypes. 2021 Apr 30 IHC ; Human, Mouse.
[IF=9.071] Lamei Xue. et al. Maternal secretin ameliorates obesity by promoting white adipose tissue browning in offspring. EMBO REP. 2022 Jul;23(7):e54132 IHC ; Mouse.
[IF=6.4] Zhang et al. The knockout of secretin in cerebellar Purkinje cells impairs mouse motor coordination and motor learning. (2014) Neuropsychopharmacolog. 39:1460-8 IHC-P ; Mouse.
[IF=0.392] Yildiz, M.. et al. The Effect of Chronic Intermittent Cold Exposure on Gastrin-, Somatostatin-, Secretin-, and Serotonin-Containing Cells in the Small Intestine of Rats. Biol Bull+. 2021 Dec;48(3):S95-S106 IHC ; Rat.
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| 研究领域 | 信号转导 生长因子和激素 内分泌病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Secretin: 28-54/121 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. Function: Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach. Subcellular Location: Secreted. Similarity: Belongs to the glucagon family. SWISS: P09683 Gene ID: 6343 Database links: Entrez Gene: 6343 Human Omim: 182099 Human SwissProt: P09683 Human Unigene: 632324 Human Secretin 促胰液素或胰泌素，由十二指肠分泌的一种多肽激素，可刺激胰液的释放。 |
| 产品图片 |
Sample:
Lane 11: Human SW480 cell lysates Lane 12: Human MCF-7 cell lysates Lane 13: Human HepG2 cell lysates Primary: Anti-Secretin (bs-0088R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13 kDa Observed band size: 13 kDa 
Tissue/cell: rat intestine tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Secrein Polyclonal Antibody, Unconjugated(bs-0088R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
