| 产品编号 | bs-0724R |
| 英文名称 | Rabbit Anti-Spindlin 1 antibody |
| 中文名称 | 卵巢癌相关蛋白抗体 |
| 别 名 | Spindlin-like protein 2; SPIN-2; DXF34; OCR; Ovarian cancer-related protein; SPIN; Spin1; SPIN1_HUMAN; Spindlin-1. |
| 研究领域 | 肿瘤 细胞生物 发育生物学 信号转导 细胞周期蛋白 细胞分化 肿瘤细胞生物标志物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Spindin 1: 3-100/237 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Spindlin 1 (Spindlin-like protein 2; SPIN-2 ) protein May play a role in cell-cycle regulation during the transition from gamete to embryo. Subcellular location in the Nucleus. Belongs to the SPIN/STSY family. Function: May play a role in cell-cycle regulation during the transition from gamete to embryo (By similarity). Subunit: Homodimer; may form higher-order oligomers. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated during oocyte meiotic maturation. Similarity: Belongs to the SPIN/STSY family. SWISS: Q9Y657 Gene ID: 10927 Database links: Entrez Gene: 10927 Human Omim: 609936 Human SwissProt: Q9Y657 Human Unigene: 146804 Human spindlin1又称：DXF34 经研究发现，可能是一种与肿瘤形成相关的原癌基因. |
| 产品图片 |
Tissue/cell: Human Cervical Cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Spindlin 1 Polyclonal Antibody, Unconjugated(bs-0724R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
