| 产品编号 | bs-1215R |
| 英文名称 | TPH Rabbit pAb |
| 中文名称 | 色氨酸羟化酶抗体 |
| 别 名 | MGC119994; TPH 1; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5 monooxygenase; Tryptophan hydroxylase 1; Tryptophan 5-hydroxylase 1; TPH1_HUMAN. |
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Specific References (5) | bs-1215R has been referenced in 5 publications.
[IF=5.561] Lei Wu. et al. Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway. FOODS. 2023 Jan;12(5):1101 WB ; Rat.
[IF=4.996] Baba, Kazuhiko. et al. Upregulation of the hypothalamo-neurohypophysial system and activation of vasopressin neurones attenuates hyperalgesia in a neuropathic pain model rat. SCI REP-UK. 2022 Jul;12(1):1-15 IHC ; Rat.
[IF=3.158] Ryo Kajihara. et al. Water intake releases serotonin from enterochromaffin cells in rat jejunal villi. 2021 Apr 28 IHC ; Rat.
[IF=2.75] Dong, Xian-Zhe, et al. "A representative prescription for emotional disease, Ding-Zhi-Xiao-Wan restores 5-HT system deficit through interfering the synthesis and transshipment in chronic mild stress-induced depressive Rats." Journal of Ethnopharmacology (2013). WB ; Rat.
[IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25. IHC-P ; rat&mink.
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| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TPH: 31-150/444 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]. Subunit: Homotetramer. Tissue Specificity: Isoform 2 seems to be less widely expressed than isoform 1. Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain. SWISS: P17752 Gene ID: 7166 Database links: Entrez Gene: 7166 Human Entrez Gene: 21990 Mouse Omim: 191060 Human SwissProt: P17752 Human SwissProt: P17532 Mouse Unigene: 591999 Human Unigene: 248684 Mouse Unigene: 94788 Rat 色氨酸在脑中首先由色氨酸羟化酶(tryoptophan hydroxylase)催化生成5羟色氨酸(5hydroxytryptophan)，再经脱羧酶作用生成5羟色胺，色氨酸羟化酶（TPH）是5-HT生物合成的限速酶，因此，色氨酸羟化酶的缺少可导致5-羟色胺的含量下降。色氨酸羟化酶主要在肝脏内代谢。 |
| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Mouse Liver tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Cerebellum tissue lysates
Primary: Anti-TPH (bs-1215R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TPH) Polyclonal Antibody, Unconjugated (bs-1215R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
