| 产品编号 | bs-1243R |
| 英文名称 | TPM2 |
| 中文名称 | 原肌球蛋白抗体 |
| 别 名 | AMCD1; Beta tropomyosin muscle; BETA-TM; DA1; DA2B; EPITHELIAL TROPOMYOSIN; FIBROBLAST MUSCLE TYPE TROPOMYOSIN; MGC109519; NEM4; TM2; TMSB; TPM2; TROP-2; TROPOMYOSIN 2; Tropomyosin 2 (beta chain); TPM2_MOUSE; TPM2_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 细胞表面分子 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 33kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TPM2: 11-130/284 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan. Function: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization. Subunit: Heterodimer of an alpha and a beta chain. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Present in primary breast cancer tissue, absent from normal breast tissue. Post-translational modifications: Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization. DISEASE: Nemaline myopathy 4 (NEM4) [MIM:609285]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Note=The disease is caused by mutations affecting the gene represented in this entry. Arthrogryposis, distal, 1A (DA1A) [MIM:108120]: A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the tropomyosin family. SWISS: P07951 Gene ID: 7169 Database links: Entrez Gene: 7169 Human Entrez Gene: 22004 Mouse Omim: 190990 Human SwissProt: P07951 Human SwissProt: P58774 Mouse Unigene: 300772 Human Unigene: 646 Mouse Unigene: 17580 Rat Trop-2蛋白主要存在于细胞胞质及表面, 它是丝裂原活化蛋白激酶(MAPK)通路的一个重要组成部，参与细胞生长、增殖、分化、死亡及细胞间的功能同步等多种生理过程. Trop-2在很多常见的肿瘤上扮演着重要角色，其中包括乳腺癌、胃癌、结肠癌、肺癌、前列腺癌、胰腺癌和子宫癌，有学者认为：Trop-2在孕期內所具有的生殖功能可能意味著它同肿瘤的生长有关联。 |
| 产品图片 |
Sample:
Small intestine (Mouse) Lysate at 40 ug Primary: Anti- TPM2 (bs-1243R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD 
Sample:
Small intestine (Mouse) Lysate at 40 ug Primary: Anti- TPM2 (bs-1243R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD 
Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TPM2 Polyclonal Antibody, Unconjugated(bs-1243R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TPM2 Polyclonal Antibody, Unconjugated(bs-1243R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
