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Rabbit Anti-APOH  antibody (bs-1570R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1570R
英文名称 APOH
中文名称 载脂蛋白H抗体
别    名 Apolipoprotein H; Activated protein C binding protein; APC inhibitor; APOH; B2G1; beta 2 glycoprotein I; beta 2 glycoprotein I precursor; BG; OTTMUSP00000003033; APOH_HUMAN.  
Specific References  (2)     |     bs-1570R has been referenced in 2 publications.
[IF=38.104] Du Xing. et al. B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis. SIGNAL TRANSDUCT TAR. 2023 Mar;8(1):1-16  Other ;  Mouse.  
[IF=4.543] Subhojit Paul. et al. Modulation of lung cytoskeletal remodeling, RXR based metabolic cascades and inflammation to achieve redox homeostasis during extended exposures to lowered pO 2. 2021 May 17  WB ;  Human, Rat.  
研究领域 心血管  免疫学  合成与降解  糖蛋白  脂蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human (predicted: Rabbit,Horse,Cow,Dog)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 36kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ApoH: 281-345/345 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Apolipoprotein H is expressed by placental trophoblast cells at high levels. Although the normal physiological role is not known, the protein appears to act as a co-factor for the binding of autoantibodies to phospholipids to trophoblasts, which is a process involved in the pathogenesis of recurrent miscarriage.

Function:
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed by the liver and secreted in plasma.

Post-translational modifications:
N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.

Similarity:
Contains 4 Sushi (CCP/SCR) domains.

SWISS:
P02749

Gene ID:
350

Database links:

Entrez Gene: 350 Human

Entrez Gene: 287774 Rat

Omim: 138700 Human

SwissProt: P02749 Human

SwissProt: P26644 Rat

Unigene: 445358 Human

Unigene: 1824 Rat



载脂蛋白H(apolipoprotein D,apoH)又称为β2糖蛋白Ⅰ(β2-glycoprotein,β2-GI),是最初是在人血浆的高密度脂蛋白中被分离出来的。apoH在结构上与其他类型的载脂蛋白存在很大的差异,被归入脂肪促成素家族。
产品图片
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-APOH (bs- 1570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-APOH (bs- 1570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Liver (Rat) Lysate at 40 ug
Lane 3: Pancreas (Rat) Lysate at 40 ug
Primary: Anti-APOH (bs-1570R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44-50 kD
Observed band size: 47 kD
Tissue/cell: human laryngeal cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOH Polyclonal Antibody, Unconjugated(bs-1570R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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