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Rabbit Anti-VEGF antibody (bs-0279R)
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说明书: 50ul  100ul  200ul
20ul/480.00元
50ul/880.00元
100ul/1580.00元
200ul/2480.00元
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产品编号 bs-0279R
英文名称 VEGF
中文名称 血管内皮生长因子抗体
别    名 Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF A Precursor.  
Specific References  (7)     |     bs-0279R has been referenced in 7 publications.
[IF=3.89] Ryzhov, Sergey, et al. "Role of a2b adenosine receptors in regulation of paracrine functions of stem cell antigen 1-positive cardiac stromal cells." Journal of Pharmacology and Experimental Therapeutics 341.3 (2012): 764-774.  Mouse.  
[IF=2.59] Zeng, K.-W., et al., Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis. Eur J Pharmacol (2013)  Mouse.  
[IF=3.17] Li, Junqin, et al. "Fatty Acid Synthase Mediates the Epithelial-Mesenchymal Transition of Breast Cancer Cells." International Journal of Biological Sciences 10.2 (2014): 171-180.  WB ;  Human.  
[IF=0.78] Zhang, Q. L., et al. "Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of liaoning cashmere goats." Genet Mol Res 12.12 (2013): 6424-6432.  IHC-P ;  Goat.  
[IF=3.41] Qi, Yuanyuan, et al. "Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells." PloS one 7.3 (2012): e33859.  WB ;  Pig.  
[IF=3.14] Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).  WB ;  Human.  
[IF=1.00] Guo, Jian-wen, et al. "Combinatorial effects of Naomai Yihao Capsules () and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tissues in rats." Journal of Traditional Chinese Medicine 32.1 (2012): 87-92.  IHC-P ;  Rat.  
研究领域 肿瘤  心血管  生长因子和激素  糖尿病  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 24kDa
细胞定位 分泌型蛋白 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human VEGF:27-120/232 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq].

Function:
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Subunit:
Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Subcellular Location:
Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

Tissue Specificity:
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.

DISEASE:
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

Similarity:
Belongs to the PDGF/VEGF growth factor family.

SWISS:
P15692

Gene ID:
7422

Database links:

Entrez Gene: 7422 Human

Entrez Gene: 22339 Mouse

Entrez Gene: 83785 Rat

Omim: 192240 Human

SwissProt: P15692 Human

SwissProt: Q00731 Mouse

SwissProt: P16612 Rat

Unigene: 73793 Human

Unigene: 282184 Mouse

Unigene: 1923 Rat





Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

生长因子和激素(Growth Factor and Hormones) 血管内皮细胞生长因子(VEGF)是一种特异作用于血管内皮细胞的多功能细胞因子,它能引起血管通透性增加,引起细胞外基质成分改变,诱导血管形成.在炎症、创伤愈合、心脏缺血、动脉粥样硬化、糖尿病性视网膜病变及肿瘤形成等与血管生成和病变有关的诸多病理过程中起重要作用.
VEGF与血管生成有关,从而也与肿瘤生长也有很大关系,近年来受到很多关注.
产品图片
Sample: Heart(Mouse) Lysate at 30 ug
Primary: Anti-VEGF (bs-0279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 25 kD
Sample: Kidney(Mouse) Lysate at 30 ug
Primary: Anti-VEGF (bs-0279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 25 kD
Sample:
heart (Mouse) Lysate at 40 ug
A549 Cell (Human) Lysate at 30 ug
Primary: Anti- VEGF (bs-0279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD
Tissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell: Hela
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit antibody (Cat#: bs-0279R) on Hela(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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