| 产品编号 | bs-0077R |
| 英文名称 | VIP |
| 中文名称 | 血管活性肠肽抗体 |
| 别 名 | Vasoactive Intestinal peptide; MGC13587; Peptide histidine methioninamide 27; PHM 27; PHM27; PHV42; VIP peptides precursor; PHI; Peptide Histidine Isoleucine; Vasoactive intestinal peptide; VIP_HUMAN. |
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Specific References (6) | bs-0077R has been referenced in 6 publications.
[IF=6.208] Zhiqiang Zhang. et al. Vasoactive Intestinal Peptide (VIP) Protects Nile Tilapia (Oreochromis niloticus) against Streptococcus agalatiae Infection. INT J MOL SCI. 2022 Jan;23(23):14895 WB ; Fish.
[IF=4.45] Wieck, Minna M., et al. "Human and murine tissue-engineered colon exhibit diverse neuronal subtypes and can be populated by enteric nervous system progenitor cells when donor colon is aganglionic." Tissue Engineering A (2015). IHC-P ;
[IF=3.508] Wieck et al. Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic. (2016) Tissue.Eng.Part.A. 22:53-64 IHC-P ; Human.
[IF=3.411] Liu Dan. et al. RSV Promotes Epithelial Neuroendocrine Phenotype Differentiation through NODAL Signaling Pathway. Biomed Res Int. 2021;2021:9956078 IF ; human.
[IF=2.85] Liang et al. Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome. (2016) Gut.Liver. 10:382-90 IHC ; Human.
[IF=1.52] Liang Q et al. Evaluation of a modified rat model for functional dyspepsia.Saudi J Gastroenterol. 2018 Jul-Aug;24(4):228-235. WB ; Rat.
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| 研究领域 | 肿瘤 心血管 免疫学 神经生物学 信号转导 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 3.3/18kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VIP: 125-152/170 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Growth Factor and Hormones VIP(Vasoactive Intestinal peptide) is a 28-amino acid peptide structurally related tosecretin. It was originally isolated from intestinal extracts and shown to be a potent vasodilator. Subsequent work demonstrated that VIP is very widely distributed in the peripheral and central nervous systems, and probably should not be considered a true GI hormone. A huge number of biological effects have been attributed to VIP. With respect to the digestive system, VIP seems to induce smooth muscle relaxation (lower esophageal sphincter, stomach, gallbladder), stimulate secretion of water into pancreatic juice and bile, and cause inhibition of gastric acid secretion and absorption from the intestinal lumen. Function: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM and PHV also cause vasodilation. Subcellular Location: Secreted. Similarity: Belongs to the glucagon family. SWISS: P01282 Gene ID: 7432 Database links: Entrez Gene: 7432 Human Entrez Gene: 22353 Mouse Omim: 192320 Human SwissProt: P01282 Human SwissProt: P32648 Mouse Unigene: 53973 Human Unigene: 98916 Mouse Unigene: 18675 Rat VIP是一个广泛分布于中枢神经系统和周围神经系统的神经肽，他有较强的血管舒张作用、支气管扩张作用、平滑肌松弛作用，具有很多免疫系统细胞的活性，是一个促分裂剂。 近年来研究表明，VIP也是一种生长因子,它对多种恶性肿瘤的发生、发展、增殖和分化起重要的促进作用，血管活性肠肽的神经保护作用受到来自胶质细胞的活性依赖的神经营养因子释放的调节。VIP和PACAP有很高的同源性。 |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VIP Polyclonal Antibody, Unconjugated (bs-0077R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
