产品编号 | bs-0628R |
英文名称 | ZNF300 |
中文名称 | 锌指蛋白300抗体 |
别 名 | Kruppel like zinc finger protein; Zinc finger protein 300; ZN300_HUMAN. |
Specific References (1) | bs-0628R has been referenced in 1 publications.
[IF=1.291] Xue et al. ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site. (2010) Cell.Mol.Biol.Lett. 15:530-40 WB ;
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研究领域 | 染色质和核信号 信号转导 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse (predicted: Human) |
产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 68kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF300: 165-250/604 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
ZNF300 contains C2H2 (or Kruppel)-type zinc finger motifs that tetrahedrally coordinate zinc ions and form a protein structure that can bind DNA and regulate transcription (Gou et al.,2004 [PubMed 14746915]). ZNF300 belongs to the krueppel C2H2 type zinc finger protein family. It contains twelve C2H2 type zinc fingers and one KRAB domain. ZNF300 has a transcriptional repressor activity. Expressed mostly in heart, skeletal muscle and brain. Isoforms 1 and 2 are highly expressed in testis. There are three named isoforms. Function: Has a transcriptional repressor activity. Subcellular Location: Nucleus. Tissue Specificity: Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed in testis. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 12 C2H2-type zinc fingers. Contains 1 KRAB domain. SWISS: Q96RE9 Gene ID: 91975 Database links: Entrez Gene: 91975 Human Omim: 612429 Human SwissProt: Q96RE9 Human Unigene: 134885 Human ZNF300白血病相关性研究。 |
产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug Primary: Anti-ZNF300 (bs-0628R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |