| 产品编号 | bs-1094R |
| 英文名称 | kappa Opioid receptor |
| 中文名称 | kappa型阿片受体抗体 |
| 别 名 | Kappa type opioid receptor; KOR 1; KOR; Opiate receptor kappa 1; OPRK 1; OPRK; OPRK1; Oprk2; R21; OPRK_HUMAN. |
| 研究领域 | 细胞生物 免疫学 神经生物学 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human kappa Opioid receptor: 201-300/380 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Opioid is the term used to designate a group of compounds that are opium-like in their properties. These drugs have effects on perception of pain, consciousness, motor control, mood, and autonomic function, and can induce physical dependence. Pharmacological studies suggested that there are at least 3 major classes of opioid receptors, designated delta, kappa, and mu. They differ in their affinity for various opioid ligands and in their cellular distribution. Studies of the receptors in the mouse and rat show that they are structurally related and are members of the family of 7 transmembrane-spanning G protein-coupled receptors.
The kappa opiod receptor inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. It is the receptor for beta-endorphin. Function: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions. Subunit: Interacts with SLC9A3R1. Interacts with GABARAPL1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P41145 Gene ID: 4986 Database links: Entrez Gene: 4986 Human Omim: 165196 Human SwissProt: P41145 Human Unigene: 106795 Human kappa型阿片受体在人脑和胎盘组织中有分布。κ- 受体参与镇痛 ，且与神经内分泌及免疫调节有关。此外，κ受体也调控喷他佐辛样脊髓镇痛、镇静和瞳孔缩小。κ-阿片受体由380个氨基酸组成，同样属于G蛋白偶联受体家族。 |
| 产品图片 |
Sample: A549 Cell Lysate at 40 ug
Primary: rabbit Anti-kappa Opioid receptor (bs-1094R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 43 kD Observed band size: 43 kD 
Sample:
Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Primary: Anti-kappa Opioid receptor (bs-1094R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 45 kDa 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (kappa Opioid receptor) Polyclonal Antibody, Unconjugated (bs-1094R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-kappa Opioid receptor Polyclonal Antibody, Unconjugated(bs-1094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
