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Rabbit Anti-GDF8 antibody (bs-1288R)
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说明书: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-1288R
英文名称 GDF8
中文名称 生长分化因子8抗体
别    名 GDF 8; GDF-8; GDF8_HUMAN; Growth differentiation factor 8; Growth/Differentiation Factor 8; MSTN; myostatin; OTTHUMP00000163498.  
研究领域 免疫学  生长因子和激素  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 12/43kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GDF8:281-375/375 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. Acts specifically as a negative regulator of skeletal muscle growth. [SUBUNIT] Homodimer; [TISSUE SPECIFICITY] Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue. Belongs to the TGF-beta family.

Function:
Acts specifically as a negative regulator of skeletal muscle growth.

Subunit:
Homodimer; disulfide-linked. Interacts with WFIKKN2, leading to inhibit its activity. Interacts with FST3.

Subcellular Location:
Secreted

Tissue Specificity:
Predominantly expressed in muscle. At hatching, expression is strongest in the skin epithelium, and is also found in the retina and brain. From day 28, expressed in skeletal muscle. In the adult, highest expression is seen in the gastrointestinal tract, brain, muscle, heart and testis. Also expressed in the adult pharynx, kidney, spleen, liver, gill, eyes, skin, swim bladder and ovary.

DISEASE:
Defects in MSTN are the cause of muscle hypertrophy (MSLHP) [MIM:614160]. MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong.

Similarity:
Belongs to the TGF-beta family.

SWISS:
O14793

Gene ID:
2660

Database links:

Entrez Gene: 2660 Human

Entrez Gene: 17700 Mouse

Entrez Gene: 373964 Chicken

Entrez Gene: 399534 Pig

Entrez Gene: 29152 Rat

Entrez Gene: 798441 Zebrafish

Omim: 601788 Human

SwissProt: O14793 Human

SwissProt: O08689 Mouse

SwissProt: O35312 Rat

SwissProt: O18830 Sheep

SwissProt: O42222 Zebrafish

Unigene: 41565 Human

Unigene: 3514 Mouse

Unigene: 44460 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

GDF-8又称MSTN,是转化生长因子超家族,也是近年来发现的一类重要的肌细胞生长调控因子,它通过抑制MyoD家族成员转录活性负向控制肌细胞的生长发育,它的表达量与肌肉重量的变化呈负相关。
产品图片
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-GDF8 (bs-1288R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/43 kD
Observed band size: 43 kD
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated(bs-1288R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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