[IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858  WB ;  Mouse.  

PubMed:37039469

[IF=4.366] Genxi Zhang. et al. MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation. Cells-Basel. 2021 Feb;10(2):423  WB ;  Chicken.  

PubMed:33671389

[IF=4.26] Qian et al. Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs. (2015) Sci.Re. 5:14435  WB ;  Pig.  

PubMed:26400270

[IF=2.788] Jin, Zhengyun. et al. Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs. TRANSGENIC RES. 2022 May;:1-9  WB ;  Pig.  

PubMed:35570234

[IF=2.59] Yamada A.K.. et al. Beta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathway. Braz J Med Biol Res. 2022 Jan;55:  WB ;  Rat.  

PubMed:35019034

Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-GDF8 (bs-1288R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/43 kD
Observed band size: 43 kD

Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Muscle (Rat) Lysate at 40 ug
Lane 3: Tongue (Rat) Lysate at 40 ug
Primary:
Anti-GDF8 (bs-1288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD

Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8 ) Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GDF8/MSTN Polyclonal Antibody, Unconjugated(bs-1288R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining