| 产品编号 | bs-1197R |
| 英文名称 | TLR5 |
| 中文名称 | Toll样受体5抗体 |
| 别 名 | toll-like receptor 5; FLJ10052; MGC126430; MGC126431; TIL 3; TIL3; TLR 5; Toll like receptor 5; Toll like receptor 5 precursor; Toll/interleukin 1 receptor like protein 3;CD285; TLR5_MOUSE. |
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Specific References (6) | bs-1197R has been referenced in 6 publications.
[IF=5.959] Shi D et al. TLR5: A prognostic and monitoring indicator for triple-negative breast cancer. Cell Death Dis. 2019 Dec 18;10(12):954. IHC ; Mouse.
[IF=4.26] Li, Chengcheng, et al. "Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway." Scientific Reports 7 (2017). WB ; Mouse.
[IF=3.829] Wang Ruibiao. et al. Effects of exosomes derived from Trichinella spiralis infective larvae on intestinal epithelial barrier function. VET RES. 2022 Dec;53(1):1-11 WB ; Pig.
[IF=3.7] Sun, Hukui, et al. "Non‐invasive imaging of allogeneic transplanted skin graft by 131I‐anti‐TLR5 mAb." Journal of Cellular and Molecular Medicine (2014). IHC-P ; Mouse.
[IF=3.42] Hao, Jing, et al. "rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner." Cellular & molecular immunology (2013). WB ; Mouse.
[IF=2.25] Sun, H., et al. "In vivo Toll‐like receptor 5 (TLR5) imaging with radiolabeled anti‐TLR5 monoclonal antibody in rapamycin‐treated mouse allogeneic skin transplantation model." Transplant Infectious Disease (2015). IHC-P ; Mouse.
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| 研究领域 | 肿瘤 免疫学 信号转导 细胞膜受体 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 95kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse TLR5: 701-810/873 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. Function: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). Subunit: Homodimer both in the absence and presence of ligand. Binds MYD88 via their respective TIR domains (By similarity). Subcellular Location: Membrane; Single-pass type I membrane protein (By similarity). Tissue Specificity: Highly expressed in liver. Detected in lung and at very low levels in most other tissues. Post-translational modifications: Phosphorylated at Tyr-799 upon flagellin binding; required for signaling (By similarity). Similarity: Belongs to the Toll-like receptor family. Contains 16 LRR (leucine-rich) repeats. Contains 1 TIR domain. SWISS: Q9JLF7 Gene ID: 53791 Database links: Entrez Gene: 7100 Human Entrez Gene: 53791 Mouse Omim: 603031 Human SwissProt: O60602 Human SwissProt: Q9JLF7 Mouse Unigene: 604542 Human Unigene: 116894 Mouse Toll样受体5可在单核细胞、未成熟树突状细胞及上皮细胞表达 ,可能是一种参与免疫应答的受体 ,其具体作用尚不清楚。 TLR5可辨认鞭毛蛋白(fla-gellin),此种蛋白可组成鞭毛,与细菌泳动有关. |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug Ovary (Mouse) Lysate at 40 ug Primary: Anti-TLR5 (bs-1197R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kD Observed band size: 130 kD 
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-TLR5 (bs-1197R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kD Observed band size: 130 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
