| 产品编号 | bs-1293R |
| 英文名称 | GABBR2 |
| 中文名称 | G氨基丁酸B型受体2抗体 |
| 别 名 | GABR2_HUMAN; Gamma-aminobutyric acid type B receptor subunit 2; GPR51; GPRC3B; GABA-B receptor 2; GABA B Receptor 2; GABA-B-R2; GABA-BR2; GABABR2; Gb2; G-protein coupled receptor 51; HG20; |
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Specific References (2) | bs-1293R has been referenced in 2 publications.
[IF=6.792] Zhao D et al. PCB52 exposure alters the neurotransmission ligand-receptors in male offspring and contributes to sex-specific neurodevelopmental toxicity. Environ Pollut.2020 Sep;264:114715. WB ; Rat.
[IF=5.62] Dan Dong. et al. Dapagliflozin inhibits the activity of lateral habenula to alleviate diabetes mellitus-induced depressive-like behavior. EXP NEUROL. 2023 Aug;366:114448 WB ; Rat.
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| 研究领域 | 细胞生物 免疫学 神经生物学 信号转导 通道蛋白 细胞膜受体 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Dog,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 103kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GABBR2: 501-650/941 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010] Function: Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Subunit: Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with ATF4 via its C-terminal region. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note=Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. Tissue Specificity: Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. Similarity: Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. SWISS: O75899 Gene ID: 9568 Database links: Entrez Gene: 9568 Human Entrez Gene: 242425 Mouse SwissProt: O75899 Human SwissProt: Q80T41 Mouse g-氨基丁酸(GABA)是哺乳动物中枢神经系统中重要的抑制性神经递质，在体内通过作用于离子通道型的GABAA、GABAC受体及代谢型的GABAB受体而发挥生理功能。 GABAB 受体存在于神经元的突触前及突触后部位，介导慢的抑制性效应，在脑内参与许多重要的生理活动和病理变化，包括认知损害、癫痫、痉挛及药物成瘾等。 GABAB2在与GABAB1形成二聚体后可激活G蛋白，但它没有结合GABA的能力；GABAB2还可增强激动剂与GABAB1的亲和力，并且通过与GABAB1形成二聚体结构而屏蔽GABAB1的胞内滞留信号。 |
| 产品图片 |
Sample:
Brain(Mouse) Lysate at 40 ug Primary: Anti-GABA B Receptor 2 (bs-1293R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103 kD Observed band size: 125 kD 
Sample:
Hela(Human) Cell Lysate at 30 ug Primary: Anti-GABA B Receptor 2 (bs-1293R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103 kD Observed band size: 125 kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABA B Receptor 2) Polyclonal Antibody, Unconjugated (bs-1293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABA B Receptor 2) Polyclonal Antibody, Unconjugated (bs-1293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
