| 产品编号 | bs-1311R |
| 英文名称 | LYVE-1 |
| 中文名称 | 淋巴管内皮透明质酸受体抗体 |
| 别 名 | LYVE1; lymphalic vessel endotheilial hyaluronan receptor 1; CRSBP 1; CRSBP1; extracellular link domain containing 1; extracellular link domain-containing 1; HAR; hyaluronic acid receptor; Cell surface retention sequence-binding protein 1; CRSBP-1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1_MOUSE; Lymphatic endothelium specific hyaluronan receptor; LYVE 1; LYVE-1; XLKD1. |
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Specific References (5) | bs-1311R has been referenced in 5 publications.
[IF=3.3] Merigo et al. Glucose transporter expression in the human colon. (2018) World.J.Gastroenterol. 24:775-793 IHC-P ; Human.
[IF=2.11] Zhang, Chun, et al. "MTDH/AEG-1-based DNA vaccine suppresses metastasis and enhances chemosensitivity to paclitaxel in pelvic lymph node metastasis."Biomedicine & Pharmacotherapy (2015). IHC-P ; Mouse.
[IF=1.935] Hayashi T et al. Characteristics of Leiomyosarcoma: Induction of Hematogenous Metastasis by Isolated Uterine Mesenchymal Tumor Stem-like Cells Anticancer Res. 2020 Mar;40(3):1255-1265. IHC ; human.
[IF=1.68] Sun, Y., et al. "A Reproducible In-vivo Model of Lymphatic Malformation in Rats." Journal of comparative pathology 145.4 (2011): 390-398. IHC-P ; Rat.
[IF=0] Michurina et al. Linagliptin alleviates fatty liver disease in diabeticdb/dbmice. (2016) World.J.Diabetes. 7:534-546 IHC ; Mouse.
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| 研究领域 | 心血管 细胞生物 免疫学 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 细胞定位 | 细胞浆 细胞膜 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse LYVE-1 terminus: 231-318/318 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. Recently a number of interesting molecules have been identified that may be exploited as markers for lymphatic endothelium, including the hyaluronan receptor LYVE1, PALE, VEGFR3, podoplanin. LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. Function: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. Subunit: Homodimer; disulfide-linked. Interacts with PDGFB and IGFBP3. Forms a transient ternary complex with PDGFB and PDGFRB in TGN. Subcellular Location: Membrane; Single-pass type I membrane protein. Note=Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. Post-translational modifications: O-glycosylated. Similarity: Contains 1 Link domain. SWISS: Q9Y5Y7 Gene ID: 114332 Database links: Entrez Gene: 10894 Human Entrez Gene: 114332 Mouse Omim: 605702 Human SwissProt: Q9Y5Y7 Human SwissProt: Q8BHC0 Mouse Unigee: 246769 Human Unigene: 655332 Human Unigene: 396078 Mouse 淋巴管标志物 LYVE-1是一种特异性氨基葡聚糖透明质酸受体，均匀分布在淋巴管的管腔面和基底膜，目前多用于肿瘤淋巴转移方面的研究。 淋巴管内皮细胞透明质酸受体1（LYVE1)LYVE1是位于淋巴管内皮细胞上的含有322个氨基酸残基的膜蛋白。与CD44糖蛋白同源，均匀分布于淋巴管内外腔面，可与细胞外基质的葡萄糖胺聚糖透明质酸结合，具有从组织摄取透明质酸盐并转运至淋巴液的作用。 |
| 产品图片 |
Sample:
Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Spleen tissue lysates Lane 3: Human Huvec cell lysates Lane 4: Human K562 cell lysates Primary: Anti- LYVE-1 (bs-1311R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 58 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
