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Rabbit Anti-ZO-1 antibody (bs-1329R)
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说明书: 50ul  100ul  200ul
20ul/580.00元
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包装/询价

产品编号 bs-1329R
英文名称 ZO-1
中文名称 胞质紧密粘连蛋白1/闭锁小带蛋白1抗体
别    名 ZO1 tight junction protein; Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1.  
Specific References  (23)     |     bs-1329R has been referenced in 23 publications.
[IF=4.12] Zou et al. Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. (2016) Sci.Rep. 6:39299  IHC ;  Mouse.  
[IF=3.97] Ruan, Zheng, et al. "Chlorogenic acid enhances intestinal barrier by decreasing MLCK expression and promoting dynamic distribution of tight junction proteins in colitic rats." Journal of Functional Foods 26 (2016): 698-708.  IHC-P ;  Rat.  
[IF=3.94] Kulhankova, Katarina, et al. "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." Infection and immunity (2017): IAI-00848.  FCM ;  Human.  
[IF=3.775] Xue Y et al. Chlorogenic acid attenuates cadmium-induced intestinal injury in Sprague–Dawley rats. Food Chem Toxicol. 2019 Aug 4;133:110751.   WB ;  Rat.  
[IF=3.743] Li N et al. Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Biomed Pharmacother. 2019 Aug;116:109044.  IHC-P ;  Mouse.  
[IF=3.73] Ruan, Zheng, et al. "Chlorogenic Acid Decreases Intestinal Permeability and Increases Expression of Intestinal Tight Junction Proteins in Weaned Rats Challenged with LPS." PLoS ONE 9.6 (2014): e97815.  IHC-P ;  Rat.  
[IF=3.571] Song M et al. Chenodeoxycholic acid (CDCA) protects against the LPS-induced impairment of intestinal epithelial barrier function via FXR-MLCK pathway. J Agric Food Chem. 2019 Aug 5.  WB ;  Pig.  
[IF=3.454] Li T et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019 May 17.  WB ;  Mouse.  
[IF=3.392] Han L et al. Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.Nutrients. 2019 Mar 20;11(3).  WB ;  Mouse.  
[IF=3.289] Chen M et al. Dietary L-Tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in Caco-2 cell monolayer model.Food Funct. 2019 May 22;10(5):2390-2398.   WB ;  Human.  
[IF=3.234] Zhuang S et al. Rhein ameliorates lipopolysaccharide-induced intestinal barrier injury via modulation of Nrf2 and MAPKs.(2019)Life Sci. Life Sci. Jan 1;216:168-175.   WB ;  Rat.  
[IF=3.118] Xia W et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019 Apr 14;72:211-217.  WB ;  Rat.  
[IF=2.86] Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512.  WB, IHC-P ;  Pig.  
[IF=2.766] Wang et al. Characteristic and functional analysis of a newly established porcine small intestinal epithelial cell line. (2014) PLoS.One. 9:e110916  WB ;  Porcine.  
[IF=2.46] Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926.  Pig.  
[IF=2.118] Li X et al. Overexpression of lncRNA H19 changes basic characteristics and affects immune response of bovine mammary epithelial cells. PeerJ. 2019 Apr 5;7:e6715.  WB ;  Bovine.  
[IF=2.03] Gu, Yan-ting, et al. "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." Neuroscience letters 493.3 (2011): 140-144.  WB ;  Rat.  
[IF=1.971] Mohlin et al. A model to study complement involvement in experimental retinal degeneration. (2018) Ups.J.Med.Sci. 123:28-42  IHC ;  porcine.  
[IF=1.851] Guo Y et al. Impaired intestinal barrier function in a mouse model of hyperuricemia. Mol Med Rep. 2019 Aug 12.   IHC-P ;  Mouse.  
[IF=0] Khuder, Saja S. Regulation of Expression of CEACAM1 and Functional Correlation in Metabolic Diseases. Diss. University of Toledo, 2016.  IHC-P ;  Mouse.  
[IF=0] Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.  IHC-P ;  Human.  
[IF=0] Chen MT et al. SIRT3 Deficiency Promotes High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease In Correlation With Impaired Intestinal Permeability Through Gut Microbial Dysbiosis. Mol. Nutr. Food Res. 2018, 1800612.  WB ;  Mouse.  
[IF=0] Bhushan A et al. MICROFLUIDIC DEVICE WITH EXTRACELLULAR MATRIX SUPPORT MEMBRANE. US Patent App. 16/270,376, 2019  ICF ;  Human.  
研究领域 细胞生物  免疫学  信号转导  细胞粘附分子  细胞表面分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig, 
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 191kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ZO-1:1551-1702/1733 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

Function:
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Subunit:
Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with CDC42BPB and MYZAP. Interacts (via PDZ domain) with GJA1.

Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells.

Tissue Specificity:
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Post-translational modifications:
Phosphorylated. Dephosphorylated by PTPRJ.

Similarity:
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain.

SWISS:
Q07157

Gene ID:
7082

Database links:

Entrez Gene: 7082 Human

Entrez Gene: 21872 Mouse

Omim: 601009 Human

SwissProt: Q07157 Human

SwissProt: P39447 Mouse

Unigene: 510833 Human

Unigene: 4342 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

胞质紧密粘连蛋白1(ZO-1)是多结构域蛋白家族膜结合鸟苷酸激酶的家族成员,在紧密连接蛋白的组成成分中起到对组织分化和器官形成方面起较重要的作用。ZO-1在包括肾、胎盘、血脑屏障等许多组织都有不同的表达,可与紧密连接上的很多跨膜蛋白相互作用。也有学者认为:ZO-1的改变与细胞通透性的增加有关。
产品图片
Independently Validated Antibody, image provided by Science Direct, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.
Black line : Positive blank control (293T); Negative blank control (HL60)
Green line : Primary Antibody (Rabbit Anti-ZO-1 antibody (bs-11320R) )
Orange line:Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
293T(Positive)and HL60(Negative control)cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody(bs-1329R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: 293T Cells(blue).
Primary Antibody: Rabbit Anti-ZO-1/AF647 Conjugated antibody (bs-1329R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-1329R-AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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