[IF=6.117] Yue Shen. et al. Lycopene prevents Di-(2-ethylhexyl) phthalate-induced mitophagy and oxidative stress in mice heart via modulating mitochondrial homeostasis. J NUTR BIOCHEM. 2023 May;115:109285  WB ;  Mouse.  

PubMed:36796548

[IF=3.974] Ning X et al.Ambient PM2. 5 causes lung injuries and coupled energy metabolic disorder. (2018) Ecotoxicol. Environ. Saf. 170  WB ;  Mouse.  

PubMed:30579162

[IF=3.95] Yan, Wei, et al. "Acute nitrogen dioxide inhalation induces mitochondrial dysfunction in rat brain." Environmental Research 138 (2015): 416-424.  WB ;  Rat.  

PubMed:25791864

[IF=3.76] Qin, Guohua, et al. "Sulfur dioxide inhalation stimulated mitochondrial biogenesis in rat brains." Toxicology 300.1 (2012): 67-74.  WB ;  Rat.  

PubMed:22677886

Sample:
Lane1:Heart (Mouse) Lysate at 30 ug
Lane2:Mucle (Mouse) Lysate at 30 ug
Primary: Anti- NRF-1/TCF11 (bs-1342R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NRF1) Polyclonal Antibody, Unconjugated (bs-1342R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NRF-1 Polyclonal Antibody, Unconjugated(bs-1342R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NRF1) polyclonal Antibody, Unconjugated (bs-1342R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control:Jurkat.
Primary Antibody (green line): Rabbit Anti-Iba1 antibody (bs-1342R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.