| 产品编号 | bs-1268R |
| 英文名称 | CCL20 Rabbit pAb |
| 中文名称 | 巨噬细胞炎性蛋白MIP-3a抗体 |
| 别 名 | CKb4; Exodus; LARC; MIP-3-alpha; MIP-3a; MIP3A; SCYA20; ST38; MIP-3[a]; exodus-1; CCL20_HUMAN; CCL20; Beta-chemokine exodus-1; CC chemokine LARC; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha (MIP-3-alpha); Small-induci |
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Specific References (4) | bs-1268R has been referenced in 4 publications.
[IF=5.714] Tianming Li. et al. IL-17D affects the chemokines and chemokine receptors of intestinal epithelial cells under hyperoxia. INT IMMUNOPHARMACOL. 2022 Dec;113:109386 IHC ; Rat, Human.
[IF=3.401] Mitchell Corey Sanders. et al. Protecting human amnion and chorion matrices (HACM) during processing: Performance enhancement in a Diabetic Mouse Model and Human Co-culture System. WOUND REPAIR REGEN. 2023 May;: IHC ; Mouse.
[IF=1.06] Qin, Shan-Yu, et al. "Role and recruitment of Th9 cells in liver cirrhosis patients." Asian Pacific Journal of Tropical Biomedicine (2016). IHC-P ; Human.
[IF=0] Liu et al. Clara cell 10-kDa protein inhibits T(H)17 responses through modulating dendritic cells in the setting of allergic rhinitis. (2013) J.Allergy.Clin.Immunol. 131:387-94.e1-12 IHC ; Mouse.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 8.6 kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCL20: 31-97/97 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Subunit: Binds to and activate CCR6. Subcellular Location: Secreted. Tissue Specificity: Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. Post-translational modifications: C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Similarity: Belongs to the intercrine beta (chemokine CC) family. SWISS: P78556 Gene ID: 6364 Database links: Entrez Gene: 6364 Human Entrez Gene: 20297 Mouse Omim: 601960 Human SwissProt: P78556 Human SwissProt: O89093 Mouse Unigene: 75498 Human Unigene: 116739 Mouse Unigene: 10722 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCL20) Polyclonal Antibody, Unconjugated (bs-1268R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
