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Rabbit Anti-HSTF2  antibody (bs-1409R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1409R
英文名称 HSTF2
中文名称 热休克转录因子2抗体
别    名 Heat shock factor protein 2; Heat shock transcription factor 2; HSF 2; HSF2; HSTF 2; HSF2_HUMAN.  
研究领域 肿瘤  细胞生物  免疫学  神经生物学  细胞凋亡  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat (predicted: Mouse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 59kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HSTF2: 401-536/536 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011].

Function:
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Subunit:
DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.

Subcellular Location:
Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation.

Similarity:
Belongs to the HSF family.

SWISS:
Q03933

Gene ID:
3298

Database links:

Entrez Gene: 3298 Human

Entrez Gene: 15500 Mouse

Omim: 140581 Human

SwissProt: Q03933 Human

SwissProt: P38533 Mouse

Unigene: 158195 Human

Unigene: 261395 Mouse

Unigene: 447362 Mouse



转录调节因子(Transcriptin Regulators)
热休克转录因子在热休克反应中的主要功能,是在热休克基因的表达过程中启动基因转录过程,促进热休克蛋白的表达.
产品图片
Sample:
HepG2 Cell(Human)Lysate at 30 ug
Primary: Anti- HSTF2 (bs-1409R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59kD
Observed band size: 59kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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