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Rabbit Anti-MTNR1B antibody (bs-0963R)
~~~促销,代码SQ220725~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0963R
英文名称 MTNR1B
中文名称 褪黑素受体1B抗体
别    名 MT2; melatonin receptor type 1B; melatonin receptor; MEL1B; MEL-1B-R; Mtnr1b; MGC129286;MGC129287; MTNR-1B; Melatonin receptor 1B; MTR1B_HUMAN.  
Specific References  (8)     |     bs-0963R has been referenced in 8 publications.
[IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551  WB,IF,IHC ;  Mouse.  
[IF=1.778] Zhao Shu-Qin. et al. The in vitro effects of melatonin and Cry gene on the secretion of estradiol from camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jan;74:106497  WB,IF ;  Camel.  
[IF=1.778] S.-Q. Zhao. et al. cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jul;76:106609  IHC ;  Camel.  
[IF=10.39] Jang, Hoon, et al. "Synergistic effect of melatonin and ghrelin in preventing cisplatin‐induced ovarian damage via regulation of FOXO3a phosphorylation and binding to the p27Kip1 promoter in primordial follicles." Journal of Pineal Research (2017).  IHC-P ;  Mouse.  
[IF=2.17] Brockus, K. E., et al. "Dietary melatonin alters uterine artery hemodynamics in pregnant Holstein heifers." Domestic Animal Endocrinology (2015).  Bovine.  
[IF=1.291] Gao Y et al. Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells.Cell Mol Biol Lett. 2019 Mar 12;24:21.  IHC-P,IF&WB ;  Mouse.  
[IF=2.478] Tabecka-Lonczynska A et al. Melatonin receptors subtypes (MT1 and MT2) in the uterus masculinus of mature male european bison. Biological and seasonal reproductive role.J Physiol Pharmacol. 2018 Feb;69(1):67-73.  WB ;  European bisons.  
[IF=2.08] Wang, S-J., et al. "Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)." Theriogenology 78.7 (2012): 1517-1526.  WB ;  Bovine.  
研究领域 细胞生物  免疫学  神经生物学  生长因子和激素  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 40kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MTNR1B: 201-300/362 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.

Function:
High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in retina and less in brain and hippocampus.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P49286

Gene ID:
4544

Database links:

Entrez Gene: 4544 Human

Omim: 600804 Human

SwissProt: P49286 Human

Unigene: 569039 Human



有学者认为:MTNR1B是组成控制褪黑激素信号通路的一部分。
褪黑激素调解人体的昼夜节律,是通过白天和黑夜来控制睡眠和饮食的内部时钟。MTNR1B的进一步研究为高血糖和糖尿病与昼夜节律遭到破坏有直接关系这一观点提供了证据。
产品图片
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Cerebellum (Rat) Lysate at 40 ug
Lane 6: Jurkat (Human) Cell Lysate at 30 ug
Lane 7: Hela (Human) Cell Lysate at 30 ug
Lane 8: U87MG (Human) Cell Lysate at 30 ug
Primary: Anti-MTNR1B (bs-0963R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/50-55 kD
Observed band size: 60/50 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- MTNR1B (bs-0963R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (bs-0963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(bs-0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(bs-0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MTNR1B) polyclonal Antibody, Unconjugated (bs-0963R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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