[IF=8.063] Hu ZB et al. Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis. Theranostics. 2020 Feb 3;10(6):2803-2816.  IHC,WB,IF ;  rat.  

PubMed:32194836

[IF=6.68] Cottle, Denny L., et al. "Fetal inhibition of inflammation improves disease phenotypes in harlequin ichthyosis." Human molecular genetics (2014): ddu459.  Mouse.  

PubMed:25209981

[IF=6.291] Changjiang Liu. et al. JNK and Jag1/Notch2 co-regulate CXCL16 to facilitate cypermethrin-induced kidney damage. ECOTOX ENVIRON SAFE. 2022 Jun;238:113582  WB,IF ;  Rat.  

PubMed:35512476

[IF=3.324] Ma et al. CXCL16 regulates renal injury and fibrosis in experimental renal artery stenosis. (2016) Am.J.Physiol.Heart.Circ.Physio. 311:H815-21  IF(IHC-F) ;  Rat.  

PubMed:27496882

[IF=2.447] Yanji Zhu. et al. P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy. Exp Ther Med. 2022 Feb;23(2):1-8  IHC ;  Mouse.  

PubMed:35069842

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CXCL16 Polyclonal Antibody, Unconjugated(bs-1441R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (bs-1441R) at 1:50 for two hours at room temperature (green channel).

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CXCL16 antibody (bs-1441R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.