产品编号 | bs-1739R |
英文名称 | Wnt1 |
中文名称 | 信号通路Wnt1抗体 |
别 名 | wingless-type MMTV integration site family, member 1; INT 1; INT1; Murine mammary tumor virus integration site 1 ; Oncogene INT1; Proto oncogene protein Wnt 1; Wnt 1; Wnt1 proto oncogene protein precursor; WNT1_HUMAN. |
Specific References (4) | bs-1739R has been referenced in 4 publications.
[IF=2.829] Sung, Hsin-Ju Chiang, et al. "Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome." American Journal of Translational Research 9.2 (2017): 214-229. IHC-P ; Rat.
[IF=2.04] Gao, Hong, et al. "Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprungs disease." International Journal of Experimental Pathology (2014). WB ; Human.
[IF=1.89] Shan, Shumei, et al. "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." Int J Clin Exp Pathol 8.10 (2015): 12357-12367. WB ; Human.
[IF=1.56] Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular medicine reports 12.2 (2015): 1971-1976. WB ; Rat.
|
|
研究领域 | 肿瘤 免疫学 神经生物学 信号转导 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Chicken,Cow,Horse) |
产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 39kDa |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Wnt1: 251-350/370 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. Function: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Subunit: Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3 (By similarity). Interacts with WLS (By similarity). Subcellular Location: Secreted, extracellular space, extracellular matrix. Post-translational modifications: Palmitoylation at Ser-224 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-93. Palmitoylation is necessary for proper trafficking to cell surface. Similarity: Belongs to the Wnt family. SWISS: P04628 Gene ID: 7471 Database links: Entrez Gene: 7471 Human Entrez Gene: 22408 Mouse Omim: 164820 Human SwissProt: P04628 Human SwissProt: P04426 Mouse Unigene: 248164 Human Unigene: 1123 Mouse Wnt1是Wnt家族的成员之一,对神经干细胞的增殖及分化有一定的调控作用,Wnt1a蛋白可以促进神经干细胞向神经元分化. |
产品图片 |
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt1 Polyclonal Antibody, Unconjugated(bs-1739R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt1 Polyclonal Antibody, Unconjugated(bs-1739R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Wnt1 Polyclonal Antibody, Unconjugated(bs-1739R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |