| 产品编号 | bs-1442R |
| 英文名称 | Arfaptin 2 Rabbit pAb |
| 中文名称 | ADP核糖基化因子结合蛋白2抗体 |
| 别 名 | ARFIP2; arfaptin 2; ADP-ribosylation factor interacting protein 2; Partner of RAC1; POR1; ARFP2_HUMAN . |
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Specific References (1) | bs-1442R has been referenced in 1 publications.
[IF=14.957] Ken Hiratsuka. et al. Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery. SCI ADV. 2022 Sep;8(38) ICC ; Human.
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| 研究领域 | 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Arfaptin 2: 221-341/341 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes. Function: Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling. Subunit: Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level. Similarity: Contains 1 AH domain. SWISS: P53365 Gene ID: 23647 Database links: Entrez Gene: 23647 Human Entrez Gene: 76932 Mouse Omim: 601638 Human SwissProt: P53365 Human SwissProt: Q8K221 Mouse Unigene: 595146 Human Unigene: 75139 Human Unigene: 41637 Mouse Unigene: 73878 Rat 交换和转运（Trafficking and Transport） |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
