[IF=5.23] Wang, Jinzi, et al. "Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus." Scientific Reports 6 (2016): 34308.  WB ;  Other Species.  

PubMed:27698384

[IF=2.08] Xing, Wen Min, et al. "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model."Environmental Toxicology and Pharmacology (2015).  WB ;  Rat.  

PubMed:26356389

Sample:
Heart(Mouse) Lysate at 40 ug
Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD

Sample:
A431(Human) Cell Lysate at 40 ug
Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD

Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: Rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Prohibitin) polyclonal Antibody, Unconjugated (bs-1449R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.