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Rabbit Anti-Caveolin-1 antibody (bs-1453R)
~~~促销,代码KT802~~~
~~~促销,代码KT0830~~~
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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-1453R
英文名称 Caveolin-1
中文名称 细胞质膜微囊蛋白-1抗体
别    名 opamine D3 receptor; Caveolin1; Caveolin 1; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1; Cav-1; ETM1; CAV 1; CAV1; CAV1_HUMAN.  
Specific References  (7)     |     bs-1453R has been referenced in 7 publications.
[IF=] Cheng S et al. Comparative analysis of Longissimus dorsi tissue from two sheep groups identifies differentially expressed genes related to growth, development and meat quality. Genomics. 2020 Sep;112(5):3322-3330.  WB ;  Sheep.  
[IF=] Zhang Q et al. CTRP13 attenuates the expression of LN and CAV-1 Induced by high glucose via CaMKKβ/AMPK pathway in rLSECs. Aging (Albany NY). 2020 Jun 17;12(12):11485-11499.  WB ;  Rat.  
[IF=8] Dixit, Saurabh, et al. "Caveolin-mediated endocytosis of the Chlamydia M278 outer membrane peptide encapsulated in poly (lactic acid)-Poly (ethylene glycol) nanoparticles by mouse primary dendritic cells enhances specific immune effectors mediated by MHC class II and CD4+ T cells." Biomaterials (2017).  IF(ICC) ;  Mouse.  
[IF=] Xian J et al. Progress in the research of p53 tumour suppressor activity controlled by Numb in triple‐negative breast cancer. J Cell Mol Med . 2020 Jul;24(13):7451-7459.  WB ;  Human.  
[IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801  WB, IF(ICC) ;  Human.  
[IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801  WB, IF(ICC) ;  Human.  
[IF=1.79] Cheng, Mao-Feng, et al. "The role of rosiglitazone in the proliferation of vascular smooth muscle cells after experimental subarachnoid hemorrhage." Acta Neurochirurgica (2014): 1-7.  IHC-P ;  Rat.  
研究领域 信号转导  细胞凋亡  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 20kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Caveolin-1:2-120/178 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq].

Function:
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.

Subunit:
Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK.

Subcellular Location:
Golgi apparatus membrane;Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.

Tissue Specificity:
Expressed in muscle and lung, less so in liver, brain and kidney.

Post-translational modifications:
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.

DISEASE:
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.

Similarity:
Belongs to the caveolin family.

SWISS:
Q03135

Gene ID:
857

Database links:

Entrez Gene: 403980 Dog

Entrez Gene: 857 Human

Entrez Gene: 12389 Mouse

Entrez Gene: 25404 Rat

Omim: 601047 Human

SwissProt: P33724 Dog

SwissProt: Q03135 Human

SwissProt: P49817 Mouse

SwissProt: P41350 Rat

Unigene: 3850 Dog

Unigene: 74034 Human

Unigene: 28278 Mouse

Unigene: 470537 Mouse

Unigene: 22518 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

信号传导(Signaling Intermediates)
Caveolin-1(小凹蛋白)是细胞生长相关信号途径及肿瘤发生发展过程中重要的抑制因子,Caveolae是细胞膜内的特殊膜结构,参与包括细胞的分子运输、细胞粘附和信号转导在内的多种细胞活动。Caveolin-1是Caveolae中重要的结构蛋白,抑制细胞生长,与多种人类肿瘤发生发展相关的信号分子相互作用。Caveolin在信号转导的整合中起支架蛋白的作用。Caveolin构成了一个蛋白家族,他们是细胞质膜中发夹样结构域的主要结构成分。Caveolin在信号转导的整合中起支架蛋白的作用。至今已经鉴定了3种Caveolin (Caveolin-1、2 和3),它们具有不同的组织分布。Caveolin-1涉及乳腺上皮细胞增生的发病机制。该抗体可识别α和β型Caveolin-1。
产品图片
Sample:
Lung (Mouse) Lysate at 40 ug
Bladder (Mouse) Lysate at 40 ug
Primary: Anti-Caveolin-1 (bs-1453R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Caveolin-1 (bs-1453R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Sample:
NIH/3T3 Cell Lysate at 40 ug
A549 Cell Lysate at 40 ug
HUVEC Cell Lysate at 40 ug
Primary: Anti-Caveolin-1 (bs-1453R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caveolin-1) Polyclonal Antibody, Unconjugated (bs-1453R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated(bs-1453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated(bs-1453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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