| 产品编号 | bs-1469R |
| 英文名称 | GRP75 |
| 中文名称 | G蛋白偶联受体75抗体 |
| 别 名 | G protein-coupled receptor75; 75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; CSA; Glucose Regulated Protein; Grp 75; GRP75; Heat shock 70kD protein 9; heat shock 70kDa protein 9; Heat shock 70kDa protein 9B; Heat shock protein 74 kDa A; Heat shock protein A; Heat shock protein cognate 74; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; MGC4500; Mortalin 2; Mortalin; Mortalin perinuclear; Mortalin2; MOT 2; MOT; MOT2; Mthsp70; p66 mortalin; P66 MOT; PBP74; Peptide binding protein 74; Stress 70 protein mitochondrial; Stress 70 protein mitochondrial precursor. |
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Specific References (4) | bs-1469R has been referenced in 4 publications.
[IF=5.396] Yi Zhao. et al. Role of mitochondria-endoplasmic reticulum coupling in lycopene preventing DEHP-induced hepatotoxicity. Food Funct. 2021 Sep;: WB ; mouse.
[IF=5.19] Peng Junjun. et al. Mitochondria-associated endoplasmic reticulum membranes participate mitochondrial dysfunction and endoplasmic reticulum stress caused by copper in duck kidney. ENVIRON SCI POLLUT R. 2023 May;:1-12 WB ; Duck.
[IF=4.155] Junjun Peng. et al. Endoplasmic reticulum-mitochondria coupling attenuates vanadium-induced apoptosis via IP3R in duck renal tubular epithelial cells. J INORG BIOCHEM. J Inorg Biochem. 2022 Apr;:111809 WB ; Duck.
[IF=3.387] Kitamura Nao. et al. Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells. J Biochem. 2021 Jul;: IF ; Mouse.
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| 研究领域 | 肿瘤 免疫学 信号转导 细胞膜受体 转运蛋白 G蛋白偶联受体 新陈代谢 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse,Human (predicted: Rabbit,Horse,Cow,Pig,Dog) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRP75: 561-679/679 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2. Function: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Subunit: Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Subcellular Location: Mitochondrion. Nucleus, nucleolus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the heat shock protein 70 family. SWISS: P38646 Gene ID: 3313 Database links: Entrez Gene: 3313 Human Entrez Gene: 15526 Mouse Omim: 600548 Human SwissProt: P38646 Human SwissProt: P38647 Mouse Unigene: 184233 Human Unigene: 209419 Mouse Unigene: 7535 Rat 信号传导（Signaling Intermediates） 葡萄糖调节蛋白75 |
| 产品图片 |
Sample:
Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Mouse Cerebrum tissue lysates Lane 4: Rat Liver tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Rat Cerebrum tissue lysates Lane 7: Rat Pancreas tissue lysates Lane 8: Human K562 cell lysates Lane 9: Human MCF-7 cell lysates Lane 10: Human Jurkat cell lysates Lane 11: Human HeLa cell lysates Lane 12: Human HepG2 cell lysates Primary: Anti-GRP75 (bs-1469R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 72 kDa 
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
