| 产品编号 | bs-0979R |
| 英文名称 | CD38 |
| 中文名称 | CD38抗体 |
| 别 名 | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38_HUMAN; CD38_MOUSE; I-19; NIM-R5 antigen. |
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Specific References (3) | bs-0979R has been referenced in 3 publications.
[IF=14.919] Jinfeng Wang. et al. Translocation of vaginal microbiota is involved in impairment and protection of uterine health. Nat Commun. 2021 Jul;12(1):1-15 IF ; Rat.
[IF=4.966] Santoshi Muppalaet al. The P387 thrombospondin‐4 variant promotes accumulation of macrophages in atherosclerotic lesions. FASEB J
. 2020 Sep;34(9):11529-11545. IHC, IF ; mouse.
[IF=1.257] Moridera K et al. Skeletal unloading reduces cluster of differentiation (CD) 38 expression in the bone marrow and osteoblasts of mice. J Orthop Sci. 2019 May 6. ICC ; Mouse.
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| 研究领域 | 肿瘤 免疫学 干细胞 糖尿病 细胞表面分子 自然杀伤细胞 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 检测分子量 | 45kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD38: 101-200/304 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Function: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. Similarity: Belongs to the ADP-ribosyl cyclase family. SWISS: P56528 Gene ID: 12494 Database links: Entrez Gene: 952 Human Entrez Gene: 12494 Mouse Omim: 107270 Human SwissProt: P28907 Human SwissProt: P56528 Mouse Unigene: 479214 Human Unigene: 249873 Mouse CD38分子是单链Ⅱ型跨膜糖蛋白，存在于很多免疫细胞表面上,CD38与无脊椎动物卵的授精、HIV感染、某些癌症以及糖尿病等有关，CD38具有许多复杂而又独特的生物学特性及功能，有调控细胞钙释放的机制。主要表达于胸腺细胞、前T和前B细胞、活化的T和B细胞、单核细胞、NK细胞、浆细胞等，可用于急性白血病的分型和活化的T细胞在自身免疫和免疫缺陷中的作用等方面的研究，也可作为生发中心B细胞的一种选择性标记。 |
| 产品图片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kD 
Sample:
Pancreas (Mouse) Lysate at 40 ug Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 48 kD 
Sample:
SP2/0(Mouse) Cell Lysate at 30 ug Primary: Anti-CD38 (bs-0979R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 54 kD 
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD38 Polyclonal Antibody, Unconjugated(bs-0979R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
