| 产品编号 | bs-1498R |
| 英文名称 | FASN |
| 中文名称 | 脂肪酸合成酶抗体 |
| 别 名 | fatty acid synthase; FASN; FAS; FA Synthase 1; MGC14367; MGC15706; OA-519; Fatty acid synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; Enoyl-[acyl-carrier-protein] reductase; Oleoyl-[acyl-carrier-protein] hydrolase; FAS_MOUSE. |
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Specific References (7) | bs-1498R has been referenced in 7 publications.
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| 研究领域 | 肿瘤 心血管 免疫学 神经生物学 信号转导 激酶和磷酸酶 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Human (predicted: Sheep,Mouse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 272kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse FASN: 651-750/2511 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation. Function: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. Subunit: Homodimer which is arranged in a head to tail fashion. Subcellular Location: Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Ubiquitous. Prominent expression in brain, lung, and liver. Similarity: Contains 1 acyl carrier domain. SWISS: P19096 Gene ID: 14104 Database links: Entrez Gene: 2194 Human Entrez Gene: 14104 Mouse Omim: 600212 Human SwissProt: P12276 Chicken SwissProt: P49327 Human SwissProt: P19096 Mouse Unigene: 8951 Chicken Unigene: 30099 Cow Unigene: 83190 Human Unigene: 236443 Mouse Unigene: 9486 Rat 合成与降解（Synthesis and Degradation） 脂肪酸合成酶fatty acid synthase是一种新的肿瘤标志物,高表达常提示肿瘤的预后不良. |
| 产品图片 |
Sample:
Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Primary: Anti-FASN (bs-1498R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 272 kDa Observed band size: 250 kDa 
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FASN Polyclonal Antibody, Unconjugated(bs-1498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
