[IF=10.19] Xingliang Dai. et al. Crosstalk between microglia and neural stem cells influences the relapse of glioblastoma in GBM immunological microenvironment. CLIN IMMUNOL. 2023 Jun;251:109333  IHC ;  Human.  

PubMed:37088298

[IF=4.372] Lin Tianji. et al. Methylmercury induces lysosomal membrane permeabilization through JNK-activated Bax lysosomal translocation in neuronal cells. Toxicol Lett. 2022 Jan;:  IF ;  Human.  

PubMed:34999165

[IF=2.438] Liru Li. et al. Rapamycin Pretreatment Alleviates Cerebral Ischemia/Reperfusion Injury in Dose-Response Manner Through Inhibition of the Autophagy and NFκB Pathways in Rats:. Dose-Response. 2020;18(3):  WB,IF ;  Rat.  

PubMed:32874166

[IF=0.18] Liu, B., et al. "Autophagy activation aggravates neuronal injury in the hippocampus of vascular dementia rats." Neural Regeneration Research 9.13 (2014): 1288.  IHC-P ;  Rat.  

PubMed:25221581

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cathepsin B (bs-1500R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/28 kD
Observed band size: 30 kD

Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin B Polyclonal Antibody, Unconjugated(bs-1500R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control: RSC96(blue).
Primary Antibody:Rabbit Anti- Cathepsin B antibody(bs-1500R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (bs-1500R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1500R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.