| 产品编号 | bs-1606R |
| 英文名称 | CYP1A1 Rabbit pAb |
| 中文名称 | 细胞色素P450 1A1抗体 |
| 别 名 | CP1A1_HUMAN; CYP1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; Hydroperoxy icosatetraenoate dehydratase; 1.14.14.1; CP1A2_HUMAN; CYP1A2; CYPIA2; Cholesterol 25-hydroxylase; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P45 |
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Specific References (4) | bs-1606R has been referenced in 4 publications.
[IF=4.932] Wenjuan Xun. et al. Resveratrol protects intestinal integrity, alleviates intestinal inflammation and oxidative stress by modulating AhR/Nrf2 pathways in weaned piglets challenged with diquat. Int Immunopharmacol. 2021 Oct;99:107989 WB ; Pig.
[IF=3.269] Jiasui Wang. et al. Aryl hydrocarbon receptor is a prognostic biomarker and is correlated with immune responses in cervical cancer. 2021 Nov 16 IHC ; Human.
[IF=3.12] Yan R et al. PM2. 5 exposure induces age-dependent hepatic lipid metabolism disorder in female mice. journal of environmental science.2019. WB ; Mouse.
[IF=2.34] Cedervall, Therése, Pia Monica Lind, and Lars Sävendahl. "Expression of the Aryl Hydrocarbon Receptor in Growth Plate Cartilage and the Impact of Its Local Modulation on Longitudinal Bone Growth." International Journal of Molecular Sciences 16.4 (2015): 8059-8069. IHC-P ; Human.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58 kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP1A1: 361-460/512 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several cofactors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. Function: Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site. Subcellular Location: Cytochrome P450 1A1: Cytoplasm. Cytochrome P450MT2A: Endoplasmic reticulum membrane. Mitochondrion membrane. Microsome membrane. Cytochrome P450MT2B: Endoplasmic reticulum. Mitochondrion. Tissue Specificity: Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary. Similarity: Belongs to the cytochrome P450 family. SWISS: P05177 Gene ID: 1544 Database links: Entrez Gene: 57491 Human Entrez Gene: 11624 Mouse Omim: 606517 Human SwissProt: A9YTQ3 Human SwissProt: Q3U1U7 Mouse Unigene: 50823 Human Unigene: 290446 Mouse Unigene: 145524 Rat 合成与降解（Synthesis and Degradation） |
| 产品图片 |
Sample:K562 Cell (Human) Lysate at 30 ug
Primary: Anti- CYP1A1 (bs-1606R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Sample:Lung (Mouse) Lysate at 40 ug
Primary: Anti- CYP1A1 (bs-1606R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP1A1) Polyclonal Antibody, Unconjugated (bs-1606R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
