| 产品编号 | bs-1778R |
| 英文名称 | GATA4 |
| 中文名称 | GATA结合蛋白4抗体 |
| 别 名 | GATA binding factor 4; GATA 4; GATA4; GATA-4; Transcription factor GATA-4; GATA-binding factor 4; Gata4; GATA4_HUMAN; GATA-binding factor 4. |
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Specific References (6) | bs-1778R has been referenced in 6 publications.
[IF=5.108] Yang Z et al. A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats. Chemosphere. 2019 Sep;231:151-160. WB ; Rat.
[IF=3.571] Wu P et al. Fluoride induces autoimmune orchitis involved with enhanced IL-17A secretion in mice testis. J Agric Food Chem. 2019 Dec 4;67(48):13333-13343. IHF ; Mouse.
[IF=3.427] Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900 IF(ICC) ; Human.
[IF=3.143] Sung-Moo Lee. et al. Effect of the phenylpyrrole fungicide fludioxonil on cell proliferation and cardiac differentiation in mouse embryonic stem cells. Reprod Toxicol. 2021 Sep;104:76 WB ; Mouse.
[IF=2.649] Kim CW et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2019 Sep 10. WB ; Mouse.
[IF=2.198] Liu, Wen. et al. Transcriptome sequencing of LMP2A-transfected gastric cancer cells identifies potential biomarkers in EBV-associated gastric cancer. VIRUS GENES. 2022 Jul;:1-12 WB ; Human.
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| 研究领域 | 心血管 免疫学 发育生物学 干细胞 转录调节因子 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Chicken,Dog,Pig,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GATA-4: 261-360/442 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. Function: Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement. Subunit: Interacts with ZNF260. Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Subcellular Location: Nucleus. Post-translational modifications: Methylation at Lys-300 attenuates transcriptional activity. DISEASE: Defects in GATA4 are the cause of atrial septal defect type 2 (ASD2) [MIM:607941]. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. ASD2 patients show other heart abnormalities including ventricular and atrioventricular septal defects, pulmonary valve thickening or insufficiency of the cardiac valves. ASD2 is not associated with defects in the cardiac conduction system or non-cardiac abnormalities. Similarity: Contains 2 GATA-type zinc fingers. SWISS: P43694 Gene ID: 2626 Database links: Entrez Gene: 2626 Human Entrez Gene: 14463 Mouse Omim: 600576 Human SwissProt: P43694 Human SwissProt: Q08369 Mouse Unigene: 243987 Human Unigene: 247669 Mouse Unigene: 26251 Rat GATA-4属于转录调节性因子，表达在心组织、胃肠道组织中，并调节该类T细胞的发育和功能。 |
| 产品图片 |
Sample: Stomach (Mouse) Lysate at 40 ug
Primary: Anti-GATA4 (bs-1778R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
