扫码关注公众号           扫码咨询技术支持           扫码咨询技术服务
  
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
产品中心-北京博奥森生物技术有限公司
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-NeuN  antibody (bs-1613R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1613R
英文名称 NeuN
中文名称 神经元核抗原抗体
别    名 RFOX3_HUMAN; RNA binding protein fox-1 homolog 3; RBFOX3; Fox-1 homolog C; Neuronal nuclei antigen (NeuN antigen); FOX3; FOX-3; HRNBP3;  
Specific References  (23)     |     bs-1613R has been referenced in 23 publications.
[IF=13.281] Yu Hei. et al. Multifunctional Immunoliposomes Enhance the Immunotherapeutic Effects of PD-L1 Antibodies against Melanoma by Reprogramming Immunosuppressive Tumor Microenvironment. 2021 Dec 16  IF,IHC ;  Mouse.  
[IF=7.69] Zhao, Wenjuan, et al. "Human APOE Genotype Affects Intraneuronal Aβ1-42 Accumulation in a Lentiviral Gene Transfer Model." Human Molecular Genetics (2013): ddt525.  Mouse.  
[IF=5.62] Derya Kaya. et al. Allopurinol attenuates repeated traumatic brain injury in old rats: A preliminary report. EXP NEUROL. 2022 Nov;357:114196  IHC ;  Rat.  
[IF=5.2] Zhao, Wenjuan, et al. "Aging reduces glial uptake and promotes extracellular accumulation of Aβ from a lentiviral vector." Frontiers in Aging Neuroscience 6 (2014): 210.  IHC-P ;  Mouse.  
[IF=5.195] Lu Hong. et al. Investigation of Naoluoxintong on the neural stem cells by facilitating proliferation and differentiation in vitro and on protecting neurons by up-regulating the expression of nestin in MCAO rats. J ETHNOPHARMACOL. 2022 Sep;:115684  WB ;  Rat.  
[IF=4.679] Junting Xiao. et al. Arsenite induces ferroptosis in the neuronal cells via activation of ferritinophagy. Food Chem Toxicol. 2021 Mar;:112114  IF ;  Mouse.  
[IF=4.545] Zhixiong Li. et al. Recovery of post-stroke cognitive and motor deficiencies by Shuxuening injection via regulating hippocampal BDNF-mediated Neurotrophin/Trk Signaling. Biomed Pharmacother. 2021 Sep;141:111828  IF ;  Mouse.  
[IF=4.074] Dingkang Xu. et al. Sphingosine‐1‐phosphate receptor 3 is implicated in BBB injury via the CCL2‐CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28  IHC ;  Human.  
[IF=3.92] Wei, Gang, et al. "β-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AβPP/PS1 mice." Journal of Alzheimer's Disease 33.3 (2013): 863-880.  IF(ICC) ;  Mouse.  
[IF=3.73] Yu, Lu, et al. "Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-κB and STAT3 in Transient Focal Stroke." PloS one 8.2 (2013): e55839.  Rat.  
[IF=3.54] Guo et al. Bone marrow-derived, neural-like cells have the characteristics of neurons to protect the peripheral nerve in microenvironment. (2015) Stem.Cells.In. 2015:941625  IF(ICC) ;  Rabbit.  
[IF=3.517] Chen YC et al. Indole Compound NC009-1 Augments APOE and TRKA in Alzheimer's Disease Cell and Mouse Models for Neuroprotection and Cognitive Improvement. J Alzheimers Dis. 2019;67(2):737-756.  IHC ;  Mouse.  
[IF=3.12] Jiang, W., et al. "Curculigoside A attenuates experimental cerebral ischemia injury< i> in vitro and< i> vivo." Neuroscience 192 (2011): 572-579.  Rat.  
[IF=2.88] Li, Hongwei, et al. "Regular treadmill running improves spatial learning and memory performance in young mice through increased hippocampal neurogenesis and decreased stress." Brain Research (2013).  Mouse.  
[IF=2.645] Xiao H et al. Osthole ameliorates cognitive impairments via augmenting neuronal population in APP/PS1 transgenic mice. Neurosci Res . 2020 Apr 14;S0168-0102(20)30008-0.  ICC,WB ;  mouse.  
[IF=2.59] Jiang, Wang-Lin, et al. "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." European journal of pharmacology 640.1 (2010): 75-81.  IHC-P ;  Rat.  
[IF=2.231] Hui Zhang. et al. MiR‐34a‐5p up‐regulates the IL‐1β/COX2/PGE2 inflammation pathway and induces the release of CGRP via inhibition of SIRT1 in rat trigeminal ganglion neurons. Febs Open Bio. 2021 Jan;11(1):300-311  IF ;  Rat.  
[IF=2.05] Kopec, Ashley M., et al. "Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue." Journal of Neuroscience Methods (2017).  WB ;  Rat.  
[IF=2] Wang, Jun, et al. "Anti‐inflammatory and retinal protective effects of capsaicin on ischemia‐induced injuries through the release of endogenous somatostatin." Clinical and Experimental Pharmacology and Physiology (2017).  IHC-P ;  Mouse.  
[IF=1.788] Bai Xue. et al. Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29. Anal Cell Pathol. 2021;2021:6622713  IF ;  Rat.  
[IF=1.56] Hu, Hai Xia, et al. "Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. Corrigendum in/mmr/12/3/3998." Molecular medicine reports 12.1 (2015): 1321-1327.  IHC-P ;  Rat.  
[IF=0.181] H Mu et al. HCMV-encoded IE2 induces anxiety-depression and cognitive impairment in UL122 genetically-modified mice. Int J Clin Exp Pathol 2019;12(11):4087-4095.  IHC-P ;  Mouse.  
[IF=0] Wang TH et al. COX5A over-expression protects cortical neurons from hypoxic ischemic injury in neonatal rats associated with TPI up-regulation. 06 January 2020, PREPRINT (Version 1) available at Research Square  IHF-P ;  Rat.  
研究领域 细胞生物  神经生物学  转运蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human (predicted: Horse,Cow,Dog)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 34kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NeuN: 51-150/312 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Function:
RNA-binding protein that regulates alternative splicing events.

Subcellular Location:
Nucleus. Cytoplasm.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
A6NFN3

Gene ID:
146713

Database links:

Entrez Gene: 146713 Human

Entrez Gene: 52897 Mouse

Entrez Gene: 287847 Rat

SwissProt: A6NFN3 Human

SwissProt: Q8BIF2 Mouse

Unigene: 135229 Human

Unigene: 341103 Mouse

Unigene: 143966 Rat



神经生物学相关蛋白(Neurobiology)
产品图片
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Primary:
Anti-NeuN (bs-1613R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/50 kD
Observed band size: 46/50 kD
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
版权所有 2004-2026 www.bioss.com.cn 北京博奥森生物技术有限公司
通过国际质量管理体系ISO 9001:2015 GB/T 19001-2016    认证编号: 00122Q31509R1M/1100
京ICP备05066980号-1         京公网安备110107000727号